Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements

doi:10.1016/j.colsurfa.2018.07.008

	Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements
	Zhang, Liang 1,2,3; Chai, Xin 1,3; Sun, Peng 3; Zhu, Qinjun 3; Zhang, Xu 3; Liu, Maili 3
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2018-10-20
卷号	555 页码:332-338
关键词	CHAPS Detergent Solvent paramagnetic relaxation enhancement Saturation transfer difference Aggregated pattern
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2018.07.008
英文摘要	CHAPS is a typical zwitterionic detergent widely used in protein purification. It has two peculiar aggregation states, whose aggregation patterns are still controversial and uncertain. In this paper, we analyzed the aggregation pattern of CHAPS by H-1 solvent Paramagnetic Relaxation Enhancement (sPRE) experiment. It was found that the H-1 sPREs of head groups of CHAPS are much smaller than those of the tail groups, and all of them become smaller simultaneously when the concentration of CHAPS is above CMC2. In addition, the sPREs of those protons in the steroid ring were found to be very similar to each other in both micellar states (M1 and M2). These results indicated that the micellar core of CHAPS is mainly formed by its steroid head which aggregate disorderly. On the other hand, a transition from single to double layer micelle is most likely to happen when the concentration of CHAPS is above CMC2. The micelle in M2 state is formed by the aggregation of the partially disordered primary ones as well. Furthermore, the STD (Saturation Transfer Difference) experiment showed that the apparent coherence transfer rates between water and those OH near protons at the steroid head are slower in M2 state, revealing that the CHAPS aggregate loosely in M1 state, but more tightly in M2 state, which may be driven by the increased hydrophobic interactions between the steroid groups of nearby CHAPS molecules, as well as the hydrogen bond between OH in the hydrophobic side and SO3-1, CO, or HN in the hydrophilic side of different molecules, which consequently extrude the water and stabilize the micelle.
资助项目	National Key R&D Program of China[2017YFA0505400] ; National Natural Science Foundation of China[21505153] ; National Natural Science Foundation of China[21120102038] ; National Natural Science Foundation of China[21675170] ; National Natural Science Foundation of China[81227902] ; National Natural Science Foundation of China[21475146] ; National Natural Science Foundation of China[21221064] ; K.C. Wong Educational Foundation
WOS关键词	BILE-SALT MICELLES ; NUCLEAR-MAGNETIC-RESONANCE ; NMR-SPECTROSCOPY ; ZWITTERIONIC DETERGENT ; CHAIN CONFORMATIONS ; AQUEOUS-SOLUTIONS ; SELF-ASSOCIATION ; BOUND PEPTIDES ; SOLUBILIZATION ; PROTEIN
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000443153100038
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; K.C. Wong Educational Foundation ; K.C. Wong Educational Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; K.C. Wong Educational Foundation ; K.C. Wong Educational Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; K.C. Wong Educational Foundation ; K.C. Wong Educational Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; K.C. Wong Educational Foundation ; K.C. Wong Educational Foundation
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13084]
专题	中国科学院武汉物理与数学研究所
通讯作者	Zhang, Xu; Liu, Maili
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Yangtze Univ, Sch Phys & Optoelect Engn, Jingzhou 430023, Peoples R China 3.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Liang,Chai, Xin,Sun, Peng,et al. Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2018,555:332-338.
APA	Zhang, Liang,Chai, Xin,Sun, Peng,Zhu, Qinjun,Zhang, Xu,&Liu, Maili.(2018).Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,555,332-338.
MLA	Zhang, Liang,et al."Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 555(2018):332-338.