UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria

doi:10.1111/1462-2920.13972

	UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria
	Chen, Min 3; Yin, Xiao-Yue 4; Zhang, Zhong-Chun 4; Shang, Jin-Long 4; Qiu, Bao-Sheng 4; Tang, Hui-Ru 1,5; Yin, Yan-Chao 4; Xu, Hai-Feng 4; Feng, Jun-Li 4; Wang, Kai 4
刊名	ENVIRONMENTAL MICROBIOLOGY
	2018
卷号	20 期号:1 页码:200-213
ISSN号	1462-2912
DOI	10.1111/1462-2920.13972
文献子类	Article
英文摘要	The small-molecule sunscreen compounds, mycosporine-like amino acids (MAAs), have strong ultraviolet (UV) absorption and can protect cyanobacteria against UV-B damage. However, the molecular mechanism underlying UV-B signaling and MAA chemical diversity remain largely unclear. Here, we identified a five-gene cluster for MAA biosynthesis in the solar radiation and desiccation tolerant cyanobacterium Nostoc flagelliforme. A LuxR family protein OrrA was identified as a positive UV-B responsive regulator binding to the promoter region of this gene cluster. OrrA functions as an activator mediating the UV-B induced MAA biosynthesis. Overexpression of orrA strengthened its UV-B tolerance during desiccation, and enhanced the photosynthetic recovery upon rehydration. Heterologous expression of this gene cluster in Anabaena PCC 7120 produces the same MAA as that in field samples of N. flagelliforme. The MAA structure is assigned as mycosporine-2-(4-deoxygadusolyl-ornithine) with a molecular weight of 756 Da, the structurally unique MAA compound reported to date. This MAA was catalyzed by mysD-mysC2-mysC1 encoding proteins from 4-deoxygadusol, which was synthesized through the catalysis of mysA-mysB products. Thus, we elucidated the transcriptional mechanism for a novel type MAA biosynthesis in solar radiation and desiccation tolerant cyanobacteria, which shed light on the identification of other components for UV-B signaling in cyanobacteria.
WOS关键词	CHLOROGLOEOPSIS PCC 6912 ; AMINO-ACIDS ; NOSTOC-FLAGELLIFORME ; PHOTOSYSTEM-II ; MICROCYSTIS-AERUGINOSA ; ULTRAVIOLET-RADIATION ; ESCHERICHIA-COLI ; COMMUNE ; NITROGEN ; PROTEIN
WOS研究方向	Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000419784100016
资助机构	National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309) ; National Natural Science Foundation of China(31670332 ; National Natural Science Foundation of China(31670332 ; 31170309) ; 31170309)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12778]
专题	中国科学院武汉物理与数学研究所
通讯作者	Qiu, Bao-Sheng
作者单位	1.Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200438, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 3.Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia 4.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China 5.Fudan Univ, Zhongshan Hosp, Collaborat Innovat Ctr Genet & Dev, Minist Educ,Key Lab Contemporary Anthropol Metabo, Shanghai 200438, Peoples R China
推荐引用方式 GB/T 7714	Chen, Min,Yin, Xiao-Yue,Zhang, Zhong-Chun,et al. UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria[J]. ENVIRONMENTAL MICROBIOLOGY,2018,20(1):200-213.
APA	Chen, Min.,Yin, Xiao-Yue.,Zhang, Zhong-Chun.,Shang, Jin-Long.,Qiu, Bao-Sheng.,...&Hao, Fu-Hua.(2018).UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria.ENVIRONMENTAL MICROBIOLOGY,20(1),200-213.
MLA	Chen, Min,et al."UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria".ENVIRONMENTAL MICROBIOLOGY 20.1(2018):200-213.