Nanobody Technology for Mycotoxin Detection: Current Status and Prospects

doi:10.3390/toxins10050180

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Nanobody Technology for Mycotoxin Detection: Current Status and Prospects
	Hu, Rui 2; Zhang, Qi 1; Li, Peiwu 1; Wang, Ting 1; Yang, Yunhuang 2; He, Ting 2; Zhu, Jiang 2; Nie, Yao 2
刊名	TOXINS
	2018-05-01
卷号	10 期号:5
关键词	Nanobody Mycotoxins Immunoassay Food Safety
DOI	10.3390/toxins10050180
文献子类	Review
英文摘要	Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. However, conventional antibodies involved in immunoassays are difficult to be expressed recombinantly and are susceptible to harsh environments. Nanobodies (or VHH antibodies) are antigen-binding sites of the heavy-chain antibodies produced from Camelidae. They are found to be expressed easily in prokaryotic or eukaryotic expression systems, more robust in extreme conditions, and facile to be used as surrogates for artificial antigens. These properties make them the promising and environmentally friendly immunoreagents in the next generation of immunoassays. This review briefly describes the latest developments in the area of nanobodies used in mycotoxin detection. Moreover, by integrating the introduction of the principle of nanobodies production and the critical assessment of their performance, this paper also proposes the prospect of nanobodies in the field of food safety in the foreseeable future.
WOS关键词	SINGLE-DOMAIN ANTIBODIES ; LINKED-IMMUNOSORBENT-ASSAY ; INDIRECT COMPETITIVE-ELISA ; MEDIATED IMMUNO-PCR ; ANTIIDIOTYPIC NANOBODY ; ENZYME-IMMUNOASSAY ; IMMUNOCHROMATOGRAPHIC ASSAY ; MONOCLONAL-ANTIBODIES ; ASPERGILLUS PATHOGENS ; AFLATOXIN B-1
WOS研究方向	Food Science & Technology ; Toxicology
语种	英语
WOS记录号	WOS:000435198800011
资助机构	National Natural Sciences Foundation of China(21575155 ; National Natural Sciences Foundation of China(21575155 ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; 31471650) ; 31471650) ; National Natural Sciences Foundation of China(21575155 ; National Natural Sciences Foundation of China(21575155 ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; 31471650) ; 31471650)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11972]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Hu, Rui,Zhang, Qi,Li, Peiwu,et al. Nanobody Technology for Mycotoxin Detection: Current Status and Prospects[J]. TOXINS,2018,10(5).
APA	Hu, Rui.,Zhang, Qi.,Li, Peiwu.,Wang, Ting.,Yang, Yunhuang.,...&Nie, Yao.(2018).Nanobody Technology for Mycotoxin Detection: Current Status and Prospects.TOXINS,10(5).
MLA	Hu, Rui,et al."Nanobody Technology for Mycotoxin Detection: Current Status and Prospects".TOXINS 10.5(2018).