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	An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain
	Tang, Xue-Ling2,5; Yuan, Qing-Song2,5; Liao, Yu-Cai2,4,5; Li, He-Ping1,2; Qu, Bo2,5; Guo, Mao-Wei2,5; Wu, Ai-Bo2,3; He, Wei-Jie1,2; Zhang, Limin6; Yi, Shu-Yuan2,5
刊名	SCIENTIFIC REPORTS
	2017-08-25
卷号	7
DOI	10.1038/s41598-017-08799-w
文献子类	Article
英文摘要	Degradation of toxins by microorganisms is a promising approach for detoxification of agricultural products. Here, a bacterial strain, Sphingomonas S3-4, that has the ability to degrade the mycotoxin deoxynivalenol (DON) was isolated from wheat fields. Incubation of Fusarium-infected wheat grains with S3-4 completely eliminated DON. In S3-4 DON is catabolized into compounds with no detectable phytotoxicity, 3-oxo-DON and 3-epi-DON, via two sequential reactions. Comparative analysis of genome sequences from two DON-degrading strains, S3-4 and Devosia D17, and one non-DON-degrading strain, Sphingobium S26, combined with functional screening of a S3-4 genomic BAC library led to the discovery that a novel aldo/keto reductase superfamily member, AKR18A1, is responsible for oxidation of DON into 3-oxo-DON. DON-degrading activity is completely abolished in a mutant S3-4 strain where the AKR18A1 gene is disrupted. Recombinant AKR18A1 protein expressed in Escherichia coli catalyzed the reversible oxidation/reduction of DON at a wide range of pH values (7.5 to 11) and temperatures (10 to 50 degrees C). The S3-4 strain and recombinant AKR18A1 also catabolized zearalenone and the aldehydes glyoxal and methyglyoxal. The S3-4 strain and the AKR18A1 gene are promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products.
WOS关键词	HEAD BLIGHT ; MYCOTOXIN DEOXYNIVALENOL ; ESCHERICHIA-COLI ; RNA GENES ; DETOXIFICATION ; WHEAT ; DEGRADATION ; RESISTANCE ; SUPERFAMILY ; ANNOTATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000408449000028
资助机构	National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; National Basic Research Program of China(2013CB127801) ; National Basic Research Program of China(2013CB127801) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Science and Technology of China(2016YFE0112900) ; Ministry of Agriculture of China(2014ZX0800202B-001) ; Ministry of Agriculture of China(2014ZX0800202B-001)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11358]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety, Res Inst Nutr Sci, Shanghai 200031, Peoples R China 4.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China 5.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 6.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Tang, Xue-Ling,Yuan, Qing-Song,Liao, Yu-Cai,et al. An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain[J]. SCIENTIFIC REPORTS,2017,7.
APA	Tang, Xue-Ling.,Yuan, Qing-Song.,Liao, Yu-Cai.,Li, He-Ping.,Qu, Bo.,...&Yi, Shu-Yuan.(2017).An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain.SCIENTIFIC REPORTS,7.
MLA	Tang, Xue-Ling,et al."An aldo-keto reductase is responsible for Fusarium toxin-degrading activity in a soil Sphingomonas strain".SCIENTIFIC REPORTS 7(2017).

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