Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice

	Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice
	Jian Li; Liang Xiang; Xiaotong Jiang; Bin Teng; Yutao Sun; Guanlian Chen; Jie Chen; Jian V.Zhang; Pei-Gen Ren
刊名	Journal OF Orthopaedic Translation
	2017
文献子类	期刊论文
英文摘要	Maintenance of healthy bone quality and quantity requires a well-coordinated balance between bone formation by osteoblasts and bone resorption by osteoclasts. Chemerin is a novel adipokine with known functions such as regulating immunity and energy homeostasis through activation of chemokine-like receptor 1 (CMKLR1). G protein-coupled receptor 1 (GPR1) is the second mammalian chemerin receptor with similar binding affinity as CMKLR1. In male GPR1–/– mice, a phenotype with significantly low bone mineral densitywas observed. We hypothesise that GPR1 might participate the process of bone remodelling. In this study, we investigated the role of GPR1 in regulating bone massmaintenance in male mice, and for the first time, revealed that GPR1–/– male mice manifested seriously trabecular bone loss and lower serum testosterone levels compared to the wild type animals. Accordingly, the mRNA expression of biomarkers related to both osteoblast [collagen type I alpha 2 (Col1A2), osteocalcin (OCN)] and osteoclast [tartrate-resistant acid phosphatase (TRAP), Cathepsin K, NFATc1] were significantly decreased or increased in GPR1–/– mice relative to the wild type, respectively. However, other osteogenic markers, Osterix and ALP levels, were increased. Microcomputed tomography scanning and histological analyses proved that there was a myriad of trabecular bone loss in GPR1–/–mice. In the meantime, GPR1–/– mice presented a significant decrease in serum testosterone level. Taken together, these findings suggested that chemerin–GPR1 signalling might be directly or indirectly communicated with testosterone synthesis on bone turnoverregulation. Further detailed studies are required to unveil how chemerin–GPR1 participates in bone metabolism.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12147]
专题	深圳先进技术研究院_医工所
作者单位	Journal OF Orthopaedic Translation
推荐引用方式 GB/T 7714	Jian Li,Liang Xiang,Xiaotong Jiang,et al. Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice[J]. Journal OF Orthopaedic Translation,2017.
APA	Jian Li.,Liang Xiang.,Xiaotong Jiang.,Bin Teng.,Yutao Sun.,...&Pei-Gen Ren.(2017).Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice.Journal OF Orthopaedic Translation.
MLA	Jian Li,et al."Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice".Journal OF Orthopaedic Translation (2017).