Mini-beam modes on standard MX beamline BL17U at SSRF
Wang, QS; Yu, F; Cui, Y; Zhang, KH; Pan, QY; Zhong, CY; Liu, K; Zhou, H; Sun, B; He, JH
刊名REVIEW OF SCIENTIFIC INSTRUMENTS
2017
卷号88期号:7页码:-
关键词Macromolecular Crystallography Beamlines Micro-crystallography Protein Crystals Diffraction Instrument Spring-8
ISSN号0034-6748
DOI10.1063/1.4991682
文献子类期刊论文
英文摘要The macromolecular crystallography beamlines at third-generation synchrotron facilities play a central role in solving macromolecular crystal structures and also in understanding the biological function at molecular levels. The MX beamline BL17U at Shanghai Synchrotron Radiation Facility is a typical standard MX beamline with a focused beam size (H x V) of FWHM around 80 mu m x 45 mu m. However the protein samples brought to the beamline are down to 5-10 m from the important and challenging science project now. These samples require smaller size beam. In order to achieve the mini-size beamline, two mini-beam modes have been developed on BL17U: the pinhole-based minibeam and the focused mini-beam by compound refractive lens (CRL). Compared to the pinhole-based mode, three times increase in flux is obtained by the CRL mode at a similar beam size. The flux gain obtained by the CRL needs to be considered for data collection strategies. It takes few minutes to switch the beamline from the normal to CRL mini-beam mode. Published by AIP Publishing.
WOS关键词MACROMOLECULAR CRYSTALLOGRAPHY BEAMLINES ; MICRO-CRYSTALLOGRAPHY ; PROTEIN CRYSTALS ; DIFFRACTION ; INSTRUMENT ; SPRING-8
语种英语
WOS记录号WOS:000406773700008
内容类型期刊论文
源URL[http://ir.sinap.ac.cn/handle/331007/28736]  
专题上海应用物理研究所_中科院上海应用物理研究所2011-2017年
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Wang, QS,Yu, F,Cui, Y,et al. Mini-beam modes on standard MX beamline BL17U at SSRF[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2017,88(7):-.
APA Wang, QS.,Yu, F.,Cui, Y.,Zhang, KH.,Pan, QY.,...&He, JH.(2017).Mini-beam modes on standard MX beamline BL17U at SSRF.REVIEW OF SCIENTIFIC INSTRUMENTS,88(7),-.
MLA Wang, QS,et al."Mini-beam modes on standard MX beamline BL17U at SSRF".REVIEW OF SCIENTIFIC INSTRUMENTS 88.7(2017):-.
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