Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification

doi:10.1021/acsami.7b01134

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification
	Li, F; Liu, XG; Zhao, B; Yan, J; Li, Q; Aldalbahi, A; Shi, JY; Song, SP; Fan, CH; Wang, LH
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017
卷号	9 期号:18 页码:15245-15253
关键词	Interaction Of Graphene Oxide And Dna Length And Position Dependent Various Dna Structures Real-time Detection Isothermal Amplification
ISSN号	1944-8244
DOI	10.1021/acsami.7b01134
文献子类	期刊论文
英文摘要	Isothermal amplification is an efficient way to amplify DNA with high accuracy; however, the real-time monitoring for quantification analysis mostly relied on expensive and precisely dsDNA designed probes. In the present study, a graphene oxide (GO)-based nanoprobe was used to real-time monitor the isothermal amplification process. The interaction between GO and different DNA structures was systematically investigated, including single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), DNA 3-helix, and long rolling circle amplification (RCA) and hybridization chain reaction (HCR) products, which existed in one-, two-, and three-dimensional structures. It was found that the high rigid structures exhibited much lower affinity with GO than soft ssDNA, and generally the rigidity was dependent on the length of targets and the hybridization position with probe DNA. On the basis of these results, we successfully monitored HCR amplification process, RCA process, and the enzyme restriction of RCA products with GO nanoprobe; other applications including the detection of the assembly/disassembly of DNA 3-helix structures were also performed. Compared to the widely used end-point detection methods, the GO-based sensing platform is simple, sensitive, cost-effective, and especially in a real-time monitoring mode. We believe such studies can provide comprehensive understandings and evocation on design of GO-based biosensors for broad application in various fields.
语种	英语
WOS记录号	WOS:000401307100005
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/27336]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Li, F,Liu, XG,Zhao, B,et al. Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(18):15245-15253.
APA	Li, F.,Liu, XG.,Zhao, B.,Yan, J.,Li, Q.,...&Wang, LH.(2017).Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification.ACS APPLIED MATERIALS & INTERFACES,9(18),15245-15253.
MLA	Li, F,et al."Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification".ACS APPLIED MATERIALS & INTERFACES 9.18(2017):15245-15253.