DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant

CORC > 化学研究所 > 中国科学院化学研究所 > 胶体、界面与化学热力学实验室

	DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant
	Fan, Yaxun 1; Wang, Hua 1,3; He, Chengqian 1; Qiao, Fulin 1,3; Wang, Shu 2; Wang, Yilin 1,3
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017-07-19
卷号	9 期号:28 页码:23333-23341
关键词	Dna Condensation Cationic Star-shaped Hexameric Surfactant Dna/surfactant Interaction Conformation Transition Cytotoxicity
英文摘要	The interactions between a star-shaped hexameric cationic quaternary ammonium surfactant PAHB and calf thymus DNA and induced DNA condensation were investigated by c-potential, dynamic light scattering, atomic force microscopy, isothermal titration calorimetry, ethidium bromide exclusion assay, circular dichroism, and cytotoxicity assay. With the addition of PAHB, long extended DNA molecules exhibit successive conformational transitions from elongated coil to a partially condensed cluster-like aggregate, to a globules-on-a-string structure, and then to a fully condensed globule until the saturation point of interaction between PAHB and DNA, which is slightly above their charge neutralization point. The efficient condensation is mainly produced by the strong attractive electrostatic interaction between the multiple positively charged headgroups of PAHB and negatively charged phosphate groups of DNA, and the hydrophobic interaction among the multiple alkyl chains of PAHB. Moreover the transition of the DNA conformation is also affected by the transitions of PAHB molecular conformation from star-shaped to claw-like and pyramid-like. Although the DNA conformation is significantly changed by PAHB, the DNA secondary structure does not display obvious variations, and the PAHB/DNA mixture does not show cytotoxicity when DNA is partially condensed. These results indicate that star-shaped oligomeric cationic surfactant is a potential condensing agent for gene transfection.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/39254]
专题	化学研究所_胶体、界面与化学热力学实验室
作者单位	1.Chinese Acad Sci, Key Lab Colloid & Interface Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Organ Solids, CAS Res Educ Ctr Excellence Mol Sci, Beijing Natl Lab Mol Sci BNLMS,Inst Chem, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fan, Yaxun,Wang, Hua,He, Chengqian,et al. DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(28):23333-23341.
APA	Fan, Yaxun,Wang, Hua,He, Chengqian,Qiao, Fulin,Wang, Shu,&Wang, Yilin.(2017).DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant.ACS APPLIED MATERIALS & INTERFACES,9(28),23333-23341.
MLA	Fan, Yaxun,et al."DNA Condensation Induced by a Star-Shaped Hexameric Cationic Surfactant".ACS APPLIED MATERIALS & INTERFACES 9.28(2017):23333-23341.