Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase

	Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase
	Liu, JingJing 1,2; Tang, Duosi 1,2,3; Chen, Zhitao 3,4; Yan, Xiaomei 1,2,3; Zhong, Zhou 1,2; Kang, Longtian 1,2; Yao, Jiannian 5
刊名	BIOSENSORS & BIOELECTRONICS
	2017-08-15
卷号	94 页码:271-277
关键词	Chemical Redox Nitrogen-doped Graphene Quantum Dots Ultrathin Cobalt Oxyhydroxide Nanosheets Alkaline Phosphatase Fluorescence
英文摘要	Alkaline phosphatase (ALP) as an essential enzyme plays an important role in clinical diagnoses and biomedical researches. Hence, the development of convenient and sensitivity assay for monitoring ALP is extremely important. In this work, on the basis of chemical redox strategy to modulate the fluorescence of nitrogen-doped graphene quantum dots (NGQDs), a novel label-free fluorescent sensing system for the detection of alkaline phosphatase (ALP) activity has been developed. The fluorescence of NGQDs is firstly quenched by ultrathin cobalt oxyhydroxide (CoOOH) nanosheets, and then restored by ascorbic acid (AA), which can reduce CoOOH to Co2+, thus the ALP can be monitored based on the enzymatic hydrolysis of L-ascorbic acid-2-phosphate (AAP) by ALP to generate AA. Quantitative evaluation of ALP activity in a range from 0.1 to 5 U/L with the detection limit of 0.07 U/L can be realized in this sensing system. Endowed with high sensitivity and selectivity, the proposed assay is capable of detecting ALP in biological system with satisfactory results. Meanwhile, this sensing system can be easily extended to the detection of various AA-involved analytes.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38501]
专题	化学研究所_光化学实验室
作者单位	1.Chinese Acad Sci, Key Lab Design & Assembly Funct Nanostruct, Fuzhou 350002, Peoples R China 2.Chinese Acad Sci, Fujian Inst Res Struct Matter, Fujian Prov Key Lab Nanomat, Fuzhou 350002, Fujian, Peoples R China 3.Fuzliou Univ, Coll Chem, Fuzhou 350002, Fujian, Peoples R China 4.Fuqing Entry Exit Inspect & Quarantine Bur, Fuqing 350002, Fujian, Peoples R China 5.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, JingJing,Tang, Duosi,Chen, Zhitao,et al. Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase[J]. BIOSENSORS & BIOELECTRONICS,2017,94:271-277.
APA	Liu, JingJing.,Tang, Duosi.,Chen, Zhitao.,Yan, Xiaomei.,Zhong, Zhou.,...&Yao, Jiannian.(2017).Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase.BIOSENSORS & BIOELECTRONICS,94,271-277.
MLA	Liu, JingJing,et al."Chemical redox modulated fluorescence of nitrogen-doped graphene quantum dots for probing the activity of alkaline phosphatase".BIOSENSORS & BIOELECTRONICS 94(2017):271-277.