Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors

	Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors
	Jiang, Jun Fang 1,2; Qiao, Juan 1,2; Mu, Xiao Yu 1,2; Moon, Myeong Hee 3; Qi, Li 1,2
刊名	TALANTA
	2017-04-01
卷号	165 页码:251-257
关键词	Magnetic Porous Polymer Membrane Covalently Immobilized Enzyme Reactor D-amino Acid Oxidase Inhibitors Screening
英文摘要	In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid-oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined (Km was 1.10 mM, V-max was 23.8 mM min(-1)) by a chiral ligand exchange capillary electrophoresis protocol with methionine as the substrate. The results indicated that the enzyme reactor could exhibit good stability and excellent reusability. Importantly, because the enzyme and the substrate could be confined into the pores of the matrices, the enzyme reactor displayed the improved enzymolysis efficiency due to the confinement effect. Further, the prepared enzyme reactor was applied for D-Amino acid oxidase inhibitors screening. It has displayed that the proposed protocol could pave a new way for fabrication of novel porous polymer membrane based enzyme reactors to screen enzyme inhibitors.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38198]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Yonsei Univ, Dept Chem, Seoul 120749, South Korea
推荐引用方式 GB/T 7714	Jiang, Jun Fang,Qiao, Juan,Mu, Xiao Yu,et al. Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors[J]. TALANTA,2017,165:251-257.
APA	Jiang, Jun Fang,Qiao, Juan,Mu, Xiao Yu,Moon, Myeong Hee,&Qi, Li.(2017).Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors.TALANTA,165,251-257.
MLA	Jiang, Jun Fang,et al."Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors".TALANTA 165(2017):251-257.