Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae

doi:10.3390/molecules22091498

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae
	Chen, Huan 1,3; Zhang, Rong 2; Luo, Rong-Hua 1; Yang, Liu-Meng 1; Wang, Rui-Rui 1; Hao, Xiao-Jiang2 ; Zheng, Yong-Tang 1,4
刊名	MOLECULES
	2017-09-01
卷号	22 期号:9 页码:1498
关键词	Antiviral Agent 12-o-tricosanoylphorbol-20-acetate Hiv Vif Apobec3g Phorbol Ester
ISSN号	1420-3049
DOI	10.3390/molecules22091498
英文摘要	APOBEC3G is a member of the human cytidine deaminase family that restricts Vif-deficient viruses by being packaged with progeny virions and inducing the G to A mutation during the synthesis of HIV-1 viral DNA when the progeny virus infects new cells. HIV-1 Vif protein resists the activity of A3G by mediating A3G degradation. Phorbol esters are plant-derived organic compounds belonging to the tigliane family of diterpenes and could activate the PKC pathway. In this study, we identified an inhibitor 12-O-tricosanoylphorbol-20-acetate (hop-8), a novel ester of phorbol which was isolated from Ostodes katharinae of the family Euphorbiaceae, that inhibited the replication of wild-type HIV-1 and HIV-2 strains and drug-resistant strains broadly both in C8166 cells and PBMCs with low cytotoxicity and the EC50 values ranged from 0.106 mu M to 7.987 mu M. One of the main mechanisms of hop-8 is to stimulate A3G expressing in HIV-1 producing cells and upregulate the A3G level in progeny virions, which results in reducing the infectivity of the progeny virus. This novel mechanism of hop-8 inhibition of HIV replication might represents a promising approach for developing new therapeutics for HIV infection.
学科主题	Chemistry, Organic
语种	英语
WOS记录号	WOS:000411499400091
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/54892]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Key Lab Bioact Peptides Yunnan Prov, Kunming 650223, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650204, Yunnan, Peoples R China 4.Soochow Univ, Coll Pharmaceut Sci, KIZ SU Joint Lab Anim Models & Drug Dev, Suzhou 215006, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Chen, Huan,Zhang, Rong,Luo, Rong-Hua,et al. Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae[J]. MOLECULES,2017,22(9):1498.
APA	Chen, Huan.,Zhang, Rong.,Luo, Rong-Hua.,Yang, Liu-Meng.,Wang, Rui-Rui.,...&Zheng, Yong-Tang.(2017).Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae.MOLECULES,22(9),1498.
MLA	Chen, Huan,et al."Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae".MOLECULES 22.9(2017):1498.