Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes

doi:10.1128/AEM.02770-06

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes
	Luo, Hong ; Liu, Yajun ; Fang, Lin ; Li, Xuan ; Tang, Ninghua ; Zhang, Keqin
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2007-06-01
卷号	73 期号:12 页码:3916-3923
关键词	Biological-activities Nematophagous Fungi Stephanocysts Nematoctonus Metabolites
ISSN号	0099-2240
DOI	10.1128/AEM.02770-06
文献子类	Article
英文摘要	We reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of Coprinus comatus (O. F. Mull.:Fr.) Pers. Although some observations regarding the role of this structure were presented, its function remained largely unknown. In this study, we showed that purified (isolated and washed) spiny balls could immobilize and kill the free-living nematode Panagrellus redivivus Goodey highly efficiently. Scanning electron microscopy studies illustrated that the spiny structure damaged the nematode cuticle, suggesting the presence of a mechanical force during the process of nematode immobilization. Severe injuries on nematode cuticles caused the leakage of inner materials of the nematodes. When these structures were ground in liquid nitrogen, their killing efficacy against nematodes was lost, indicating that the shape and the complete structure of the spiny balls are indispensable for their function. However, extraction with organic solvents never lowered their activity against P. redivivus, and the extracts showed no obvious effect on the nematode. We also investigated whether C comatus was able to produce toxins which would aid in the immobilization of nematodes. In total, we identified seven toxins from C. comatus that showed activity to immobilize the nematodes P. redivivus and Meloidogyne incognita (Kofoid et White) Chitwood. The chemical structures of these toxins were identified with nuclear magnetic resonance, mass spectrometry, infrared, and LTV spectrum analysis. Two compounds were found to be novel. The toxins found in C. comatus are O-containing heterocyclic compounds.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
WOS关键词	BIOLOGICAL-ACTIVITIES ; NEMATOPHAGOUS FUNGI ; STEPHANOCYSTS ; NEMATOCTONUS ; METABOLITES
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000247394200018
公开日期	2011-11-24
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/2071]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan 650091, Peoples R China 2.Yunnan Normal Univ, Middle Sch Attached, Yunnan 650106, Peoples R China 3.Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Kunming 650093, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Luo, Hong,Liu, Yajun,Fang, Lin,et al. Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(12):3916-3923.
APA	Luo, Hong,Liu, Yajun,Fang, Lin,Li, Xuan,Tang, Ninghua,&Zhang, Keqin.(2007).Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(12),3916-3923.
MLA	Luo, Hong,et al."Coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.12(2007):3916-3923.