Melissoidesin G, a diterpenoid purified from Isodon melissoides, induces leukemic-cell apoptosis through induction of redox imbalance and exhibits synergy with other anticancer agents

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Melissoidesin G, a diterpenoid purified from Isodon melissoides, induces leukemic-cell apoptosis through induction of redox imbalance and exhibits synergy with other anticancer agents
	Yu, Zu-Yin ; Liang, Yu-Guang ; Xiao, He ; Shan, Ya-Jun ; Dong, Bo ; Huang, Rui ; Fu, Ya-Li ; Zhao, Zhen-Hu ; Liu, Ze-Yuan ; Zhao, Qin-Shi
刊名	INTERNATIONAL JOURNAL OF CANCER
	2007-11-01
卷号	121 期号:9 页码:2084-2094
关键词	Melissoidesin g Leukemia Apoptosis Redox Arsenic Trioxide
ISSN号	0020-7136
英文摘要	Melissoidesin G (MOG) is a new diterpenoid purified from Isodon melissoides, a plant used in Chinese traditional medicine as antitumor and anti-inflammatory agents. In our study, MOG was shown to specifically inhibit the growth of human leukemia cell lines and primary acute myeloid leukemia (AML) blasts via induction of apoptosis, with the evidence of mitochondrial Delta Psi m loss, reactive oxygen species production, caspases activation and nuclear fragmentation. Furthermore, it was shown that thiol-containing antioxidants completely blocked MOG-induced mitochondrial Delta Psi m loss and subsequent cell apoptosis, while the inhibition of apoptosis by benzyloxy-carbonyl-Val-Ala-Asp-fluoromethylketone only partially attenuated mitochondrial Delta Psi m loss, indicating that MOG-induced redox imbalance is an early event upstream to mitochondrial Delta Psi m loss and caspase-3 activation. Consistently, it was found that MOG rapidly decreased the intracellular glutathione (GSH) content in a dose-dependent manner and the significance of GSH depletion in MOG-induced apoptosis was further supported by the protective effects of tert-butyl hvdroquinone (tBHQ) and the facilitative effects of DL-buthionine (S,R)-sulfoximine (BSO). Furthermore, it was showed that GSH depletion induced by MOG rendered some leukemia cell lines more sensitive to arsenic trioxide (As2O3), doxorubicin or cisplatin. Additionally, the synergistic apoptotic effects of 1106 with As2O3 were detected in HL-60 and primary AML cells, but not in normal cells, suggesting the selective toxicity of their combination to the malignant cells. Together, we proposed that MOG alone or administered with other anticancer agents may provide a novel therapeutic strategy for leukemia. (C) 2007 Wiles-Liss. Inc.
语种	英语
WOS记录号	WOS:000249718100026
公开日期	2011-11-24
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/1683]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Beijing Inst Radiat Med, Dept Pathophysiol, Beijing 100850, Peoples R China 2.Beijing 307 Hosp, Acad Med Sci, Dept Clin Pharmacol, Beijing, Peoples R China 3.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Mol Immunol, Beijing 100730, Peoples R China 4.Chinese Acad Med Sci, Kunming Inst Bot, Dept Phytochem, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Zu-Yin,Liang, Yu-Guang,Xiao, He,et al. Melissoidesin G, a diterpenoid purified from Isodon melissoides, induces leukemic-cell apoptosis through induction of redox imbalance and exhibits synergy with other anticancer agents[J]. INTERNATIONAL JOURNAL OF CANCER,2007,121(9):2084-2094.
APA	Yu, Zu-Yin.,Liang, Yu-Guang.,Xiao, He.,Shan, Ya-Jun.,Dong, Bo.,...&Cong, Yu-Wen.(2007).Melissoidesin G, a diterpenoid purified from Isodon melissoides, induces leukemic-cell apoptosis through induction of redox imbalance and exhibits synergy with other anticancer agents.INTERNATIONAL JOURNAL OF CANCER,121(9),2084-2094.
MLA	Yu, Zu-Yin,et al."Melissoidesin G, a diterpenoid purified from Isodon melissoides, induces leukemic-cell apoptosis through induction of redox imbalance and exhibits synergy with other anticancer agents".INTERNATIONAL JOURNAL OF CANCER 121.9(2007):2084-2094.