Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews
	Li, Guoping 1,2,3; Luo, Huairong4 ; Sun, Guotao 3; Wu, Guisheng; Wu, Gang ; Wang, Yan 1,2; Man, Yong 1,2; Wang, Shu 1,2; Li, Jian 1,2; Chen, Baosheng 3
刊名	MOLECULAR BIOLOGY REPORTS
	2013-09-01
卷号	40 期号:9 页码:5429-5438
关键词	Apolipoprotein Av Cdna Sequence Hdl-c Recombinant Protein Tree Shrew Triglyceride
ISSN号	0301-4851
通讯作者	Li, J (reprint author), Beijing Hosp, Key Lab Geriatr, Beijing 100730, Peoples R China. ; lijian@bjhmoh.cn ; bschen@ibms.pumc.edu.cn
文献子类	Article
英文摘要	Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to atherosclerosis, we performed apoAV cloning and characterization. The full-length cDNA of apoAV was identified using SMART-RACE. ApoAV cDNA sequence revealed two transcripts, 1,948 and 1,397 base pairs, due to alternative polyadenylation. These two transcripts share the same open reading frame (ORF), which encodes a 369-amino acid protein with high identity to human apoAV (75 %), including a 23-amino acid N-terminal signal peptide. ApoAV is expressed exclusively in the liver. Mature apoAV was expressed in E. coli BL21(DE3) and purified by Ni-chelated resin. Lipoprotein lipase activity was significantly stimulated by this recombinant protein. The full-length ORF of apoAV was cloned into pDsRed-monomer-N1 vector with a red fluorescent protein tag and was primarily localized in cytoplasm of hepG2 cells. The successful cloning, expression and localization of apoAV in tree shrew has laid down the foundation for further investigation on its structure and functions.
学科主题	Biochemistry & Molecular Biology
WOS关键词	PLASMA TRIGLYCERIDE ; V DEFICIENCY ; SERUM ; VLDL ; MICE ; A5 ; HYPERTRIGLYCERIDEMIA ; IDENTIFICATION ; LIPOPROTEINS ; BINDING
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000323504000021
资助机构	National Basic Research Program of China [2012CB517502, 2006CB503801]; National Natural Science Foundation of China [81270948, 81070634, 81170381]; Beijing Hospital Youth Initial Funding [BJ-2011-30]
公开日期	2013-10-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/16827]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Beijing Hosp, Key Lab Geriatr, Beijing 100730, Peoples R China 2.Minist Hlth, Beijing Inst Geriatr, Beijing 100730, Peoples R China 3.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci,Natl Lab Med Mol Biol, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100005, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li, Guoping,Luo, Huairong,Sun, Guotao,et al. Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews[J]. MOLECULAR BIOLOGY REPORTS,2013,40(9):5429-5438.
APA	Li, Guoping.,Luo, Huairong.,Sun, Guotao.,Wu, Guisheng.,Wu, Gang.,...&Chen, Baosheng.(2013).Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews.MOLECULAR BIOLOGY REPORTS,40(9),5429-5438.
MLA	Li, Guoping,et al."Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews".MOLECULAR BIOLOGY REPORTS 40.9(2013):5429-5438.