A clustering approach to multireference alignment of single-particle projections in electron microscopy

doi:10.1016/j.jsb.2010.03.011

	A clustering approach to multireference alignment of single-particle projections in electron microscopy
	Sorzano, C. O. S.1; Bilbao-Castro, J. R.2; Shkolnisky, Y.3; Alcorlo, M.1; Melero, R.1; Caffarena-Fernandez, G.4; Li, M.5; Xu, G.5; Marabini, R.6; Carazo, J. M.1
刊名	JOURNAL OF STRUCTURAL BIOLOGY
	2010-08-01
卷号	171 期号:2 页码:197-206
关键词	Single-particle analysis 2D analysis Multireference analysis Electron microscopy
ISSN号	1047-8477
DOI	10.1016/j.jsb.2010.03.011
英文摘要	Two-dimensional analysis of projections of single-particles acquired by an electron microscope is a useful tool to help identifying the different kinds of projections present in a dataset and their different projection directions. Such analysis is also useful to distinguish between different kinds of particles or different particle conformations. In this paper we introduce a new algorithm for performing two-dimensional multireference alignment and classification that is based on a Hierarchical clustering approach using correntropy (instead of the more traditional correlation) and a modified criterion for the definition of the clusters specially suited for cases in which the Signal-to-Noise Ratio of the differences between classes is low. We show that our algorithm offers an improved sensitivity over current methods in use for distinguishing between different projection orientations and different particle conformations. This algorithm is publicly available through the software package Xmipp. (C) 2010 Elsevier Inc. All rights reserved.
资助项目	European Union[FP6-502828] ; European Union[UE-512092] ; 3DEM European network[LSHG-CT-2004-502828] ; ANR[PCV06-142771] ; Spanish Ministerio de Educacion[CSD2006-0023] ; Spanish Ministerio de Educacion[BIO2007-67150-001] ; Spanish Ministerio de Educacion[BIO2007-67150-C03] ; Spanish Ministerio de Ciencia e Innovacion[ACI2009-1022] ; Spanish Fondo de Investigacion Sanitaria[04/0683] ; Comunidad de Madrid[S-GEN-0166-2006] ; European Social Fund ; Ministerio de Ciencia e Innovacion ; NSFC[60773165] ; NSFC[10990013] ; National Heart, Lung, And Blood Institute[R01HL070472] ; [MCI-TIN2008-01117] ; [JA-P06-TIC01426]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000279151900010
内容类型	期刊论文
源URL	[http://ir.amss.ac.cn/handle/2S8OKBNM/11196]
专题	中国科学院数学与系统科学研究院
通讯作者	Sorzano, C. O. S.
作者单位	1.CSIC, Biocomp Unit, Natl Biotechnol Ctr, Madrid 28049, Spain 2.Univ Almeria, Comp Architecture & Elect Dept, Almeria, Spain 3.Tel Aviv Univ, Dept Appl Math, IL-69978 Tel Aviv, Israel 4.Univ San Pablo CEU, Dept Ingn Sistemas Elect & Telecomunicac, Madrid, Spain 5.Chinese Acad Sci, Inst Computat Math & Sci Engn Comp, Beijing, Peoples R China 6.Univ Autonoma Madrid, Dept Comp Sci, Madrid, Spain
推荐引用方式 GB/T 7714	Sorzano, C. O. S.,Bilbao-Castro, J. R.,Shkolnisky, Y.,et al. A clustering approach to multireference alignment of single-particle projections in electron microscopy[J]. JOURNAL OF STRUCTURAL BIOLOGY,2010,171(2):197-206.
APA	Sorzano, C. O. S..,Bilbao-Castro, J. R..,Shkolnisky, Y..,Alcorlo, M..,Melero, R..,...&Carazo, J. M..(2010).A clustering approach to multireference alignment of single-particle projections in electron microscopy.JOURNAL OF STRUCTURAL BIOLOGY,171(2),197-206.
MLA	Sorzano, C. O. S.,et al."A clustering approach to multireference alignment of single-particle projections in electron microscopy".JOURNAL OF STRUCTURAL BIOLOGY 171.2(2010):197-206.