Mechanism of the allosteric regulation of Streptococcus mutans 2 '-deoxycytidylate deaminase

doi:10.1107/S2059798316009153

	Mechanism of the allosteric regulation of Streptococcus mutans 2 '-deoxycytidylate deaminase
	Dong YH(董宇辉); Li YH(李艳华); Gao ZQ(高增强); Li, YH; Guo, Z; Jin, L; Wang, DQ; Gao, ZQ; Su, XD; Hou, HF
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
	2016
卷号	72 期号:7 页码:883-891
关键词	allosteric regulation crystal structure enzyme inactivation enzyme mechanism enzyme structure 2 '-deoxycytidylate deaminase Streptococcus mutans
DOI	10.1107/S2059798316009153
通讯作者	董宇辉
英文摘要	In cells, dUMP is the intermediate precursor of dTTP in its synthesis during deoxynucleotide metabolism. In Gram-positive bacteria and eukaryotes, zinc-dependent deoxycytidylate deaminases (dCDs) catalyze the conversion of dCMP to dUMP. The activity of dCD is allosterically activated by dCTP and inhibited by dTTP. Here, the crystal structure of Streptococcus mutans dCD (SmdCD) complexed with dTTP is presented at 2.35 angstrom resolution, thereby solving the first pair of activator-bound and inhibitor-bound structures from the same species to provide a more definitive description of the allosteric mechanism. In contrast to the dTTP-bound dCD from the bacteriophage S-TIM5 (S-TIM5-dCD), dTTP-bound SmdCD adopts an inactive conformation similar to the apo form. A structural comparison suggests that the distinct orientations of the triphosphate group in S-TIM5-dCD and SmdCD are a result of the varying protein binding environment. In addition, calorimetric data establish that the modulators bound to dCD can be mutually competitively replaced. The results reveal the mechanism underlying its regulator-specific activity and might greatly enhance the understanding of the allosteric regulation of other dCDs.
语种	英语
WOS记录号	WOS:000379912500007
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/260448]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Dong YH,Li YH,Gao ZQ,et al. Mechanism of the allosteric regulation of Streptococcus mutans 2 '-deoxycytidylate deaminase[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,2016,72(7):883-891.
APA	董宇辉.,李艳华.,高增强.,Li, YH.,Guo, Z.,...&Dong, YH.(2016).Mechanism of the allosteric regulation of Streptococcus mutans 2 '-deoxycytidylate deaminase.ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY,72(7),883-891.
MLA	董宇辉,et al."Mechanism of the allosteric regulation of Streptococcus mutans 2 '-deoxycytidylate deaminase".ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72.7(2016):883-891.