Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule

doi:http://dx.doi.org/10.1007/s12195-017-0477-8

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	Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule
	Geng, YZ; Jin, YM; Lu, LN; Ma, YL; Lu, G; Zhang, H; Ji, Q; Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.
刊名	CELLULAR AND MOLECULAR BIOENGINEERING
	2017
卷号	10 期号:2 页码:162-173
关键词	Kinesin Microtubule Nucleotide-binding Pocket Anchor Effect
DOI	http://dx.doi.org/10.1007/s12195-017-0477-8
英文摘要	Microtubule not only provides the track for kinesin but also modulates kinesin's mechanochemical cycle. Microtubule binding greatly increases the rates of two chemical steps occurring inside the nucleotide-binding pocket (NBP) of kinesin, i.e., ATP hydrolysis and ADP release. Kinesin neck linker docking (the key force-generation step) is initiated by the motor head rotation induced by ATP binding which needs an anchor provided by microtubule. These functions of microtubule can only be accomplished through interactions with kinesin. Based on the newly obtained crystal structures of kinesin-microtubule complexes, we investigate the interactions between kinesin's NBP and microtubule using molecular dynamics simulations. We find that the N-3 motif of NBP has direct interactions with a group of negatively charged residues on alpha-tubulin through Ser235 and Lys237. These specific long-range interactions induce binding of NBP to microtubule at the right position and assist the formation of the indirect interaction between NBP and microtubule. These interactions between N-3 and microtubule have an important anchor effect for kinesin's motor domain during its rotation with Ser235 as the rotation center, and also play a crucial role in stabilizing the ATP-hydrolysis environment.
学科主题	Cell Biology ; Biophysics
语种	英语
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/22094]
专题	理论物理研究所_理论物理所1978-2010年知识产出
通讯作者	Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Geng, YZ,Jin, YM,Lu, LN,et al. Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule[J]. CELLULAR AND MOLECULAR BIOENGINEERING,2017,10(2):162-173.
APA	Geng, YZ.,Jin, YM.,Lu, LN.,Ma, YL.,Lu, G.,...&Ji, Q .(2017).Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule.CELLULAR AND MOLECULAR BIOENGINEERING,10(2),162-173.
MLA	Geng, YZ,et al."Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule".CELLULAR AND MOLECULAR BIOENGINEERING 10.2(2017):162-173.