Temperature dependence of circular DNA topological states

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	Temperature dependence of circular DNA topological states
	Chen, Hu; Liu, Yanhui; Zhou, Zhen; Hu, Lin; Ou-Yang, Zhong-Can; Yan, Jie; Chen, H , Natl Univ Singapore, Dept Phys, 2 Sci Dr 3, Singapore 117542, Singapore
刊名	PHYSICAL REVIEW E
	2009
卷号	79 期号:4 页码:-
关键词	Double Helix Entropic Elasticity Free-energy Cyclization Fluctuations Simulations Kinking Length Twist
ISSN号	1539-3755
英文摘要	Circular double-stranded DNA has different topological states which are defined by their linking numbers. Equilibrium distribution of linking numbers can be obtained by closing a linear DNA into a circle by ligase. Using Monte Carlo simulation, we predict the temperature dependence of the linking number distribution of small circular DNAs. Our predictions are based on flexible defect excitations that resulted from local melting or unstacking of DNA base pairs. We found that the reduced bending rigidity alone can lead to measurable changes of the variance of linking number distribution of short circular DNAs. If the defect is accompanied by local unwinding, the effect becomes much more prominent. The predictions can be easily investigated in experiments, providing a new method to study the micromechanics of sharply bent DNAs and the thermal stability of specific DNA sequences. Furthermore, the predictions are directly applicable to the studies of binding of DNA-distorting proteins that can locally reduce DNA rigidity, form DNA kinks, or introduce local unwinding.
学科主题	Physics
URL标识	查看原文
WOS记录号	WOS:000265941300120
公开日期	2012-08-02
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/5350]
专题	理论物理研究所_理论物理所1978-2010年知识产出
通讯作者	Chen, H , Natl Univ Singapore, Dept Phys, 2 Sci Dr 3, Singapore 117542, Singapore
推荐引用方式 GB/T 7714	Chen, Hu,Liu, Yanhui,Zhou, Zhen,et al. Temperature dependence of circular DNA topological states[J]. PHYSICAL REVIEW E,2009,79(4):-.
APA	Chen, Hu.,Liu, Yanhui.,Zhou, Zhen.,Hu, Lin.,Ou-Yang, Zhong-Can.,...&Chen, H , Natl Univ Singapore, Dept Phys, 2 Sci Dr 3, Singapore 117542, Singapore.(2009).Temperature dependence of circular DNA topological states.PHYSICAL REVIEW E,79(4),-.
MLA	Chen, Hu,et al."Temperature dependence of circular DNA topological states".PHYSICAL REVIEW E 79.4(2009):-.