Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)

	Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)
	Zhang, Dong-Yuan 1,2; Chen, Na 1,2; Yang, Yan-Zhuo 1,2; Zhang, Qian 1,2; Liu, Jian-Quan 1,2
刊名	conservation genetics
	2008
卷号	9 期号:2 页码:475-477
关键词	rhubarb Rheum tanguticum microsatellite markers genetic diversity conservation
合作状况	其它
中文摘要	rhubarb is an important traditional chinese medicine. however, the wild resource has been declining. in order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. here, we describe the development of 10 new microsatellite loci for ac/tg/cca in rheum tanguticum. the microsatellites were enriched using the combined biotin capture method. the polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. the number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. all markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with r. tanguticum. these microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species.
英文摘要	rhubarb is an important traditional chinese medicine. however, the wild resource has been declining. in order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. here, we describe the development of 10 new microsatellite loci for ac/tg/cca in rheum tanguticum. the microsatellites were enriched using the combined biotin capture method. the polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. the number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. all markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with r. tanguticum. these microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species.
学科主题	遗传学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biodiversity conservation ; genetics & heredity
研究领域[WOS]	biodiversity & conservation ; genetics & heredity
关键词[WOS]	morphology
收录类别	SCI
语种	英语
WOS记录号	WOS:000254242400026
公开日期	2009-12-07
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1227]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Lanzhou Univ, Sch Life Sci, MOE Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,et al. Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)[J]. conservation genetics,2008,9(2):475-477.
APA	Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,Zhang, Qian,&Liu, Jian-Quan.(2008).Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae).conservation genetics,9(2),475-477.
MLA	Zhang, Dong-Yuan,et al."Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)".conservation genetics 9.2(2008):475-477.