Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2

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	Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2
	Li YC(李艳春); Zhang, Cai ; Yang, Liancheng ; Ma, Enlong ; Liu LQ(刘连庆); Liu M(刘苗); Xu, Hanying ; Zhang, Ling
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2018
卷号	495 期号:1 页码:325-331
关键词	Pulmonary fibrosis MRC-5 Salvianolic acid B Oxidative stress Nrf2
ISSN号	0006-291X
通讯作者	Liu M(刘苗) ; Li YC(李艳春)
产权排序	1
中文摘要	Salvianolic acid B (SalB) is one of the most bioactive components extracted from Salvia miltiorrhiza, and its antioxidant capacity corresponds with its protective effects against cell injury from oxidative stress. The aim of the present study was to evaluate the effect of SalB on experimental pulmonary fibrosis and its ability to ameliorate the oxidative/antioxidative imbalance during fibrosis pathogenesis. The anti fibrotic activity of SalB was first confirmed in Transforming growth factor beta 1(TGF-beta 1)-stimulated MRC-5 cells. The protection of SalB against oxidative stress during fibrogenesis in vitro was verified by detecting ROS production, the levels of glutathione (GSH) and malondialdehyde (MDA). The Western blot and PCR results indicated that SalB could up-regulate nuclear factor erythroid-derived 2-like 2 (Nrf2) at both the protein and mRNA levels and induce Nrf2 nuclear translocation in vitro, which may be the mechanism underlying the anti-fibrotic capacity of SalB. Furthermore, the anti-fibrotic and antioxidant capacities of SalB in vivo were confirmed in rats with BLM-induced pulmonary fibrosis. The immunohistochemistry results showed that Nrf2 was absent in fibroblastic foci (FF) areas, while the SalB treatment could increase the expression of Nrf2 in lung tissues, especially in FF areas. (C) 2017 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	OXIDATIVE STRESS
收录类别	SCI
语种	英语
WOS记录号	WOS:000423897600051
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/21530]
专题	沈阳自动化研究所_机器人学研究室
作者单位	1.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences, Shenyang 110016, China 3.Research Institute of Petrochemical Technology, Liaoning Petrochemical Vocational and Technology College, Jinzhou 121001, China
推荐引用方式 GB/T 7714	Li YC,Zhang, Cai,Yang, Liancheng,et al. Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(1):325-331.
APA	Li YC.,Zhang, Cai.,Yang, Liancheng.,Ma, Enlong.,Liu LQ.,...&Zhang, Ling.(2018).Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(1),325-331.
MLA	Li YC,et al."Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.1(2018):325-331.