Patterned microwell arrays for single-cell analysis & drug screening

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	Patterned microwell arrays for single-cell analysis & drug screening
	Yang WG(杨文广); Liu LQ(刘连庆); Wang YC(王越超); Yu HB(于海波)
	2017
会议名称	1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017
会议日期	July 17-21, 2017
会议地点	Montreal, Canada
关键词	micro-well arrays microfabrication single-cell drug screening
页码	1-5
通讯作者	Yu HB(于海波) ; Liu LQ(刘连庆)
中文摘要	Nowadays, cells are commonly used for predicting whether the drug compounds will be effective and safe in human. The response of cell groups was usually analyzed, instead of single cell. However, individual cells within the same population even shows various characteristics. Understanding this heterogeneity is critical to studying how effective therapies will be in the clinic. Therefore, highly efficient analysis of single cell has become important for cellular biologists for both fundamental and drug screening. Recently, several methods including microscopy, laser capture microdissection, flow cytometry and microfluidic techniques have been developed for study the single cell. However, miniaturization, simplification and multifunction are still main challenges for the development of single cell analytical tool. In this paper, we have presented an easy-to-use and versatile method to fabricate various microwells for single cell analysis. Cells could grow into arbitrary shapes with constrained microwells. The microwells were able to be removed to investigate cell shapes effect on growth via our method. Furthermore, 3D multicellular spheroids which can be used for dug screening were formed based on micro-wells arrays.
收录类别	EI
产权排序	1
会议录	International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
会议录出版者	IEEE
会议录出版地	New York
语种	英语
ISBN号	978-1-5386-0346-8
内容类型	会议论文
源URL	[http://ir.sia.cn/handle/173321/21072]
专题	沈阳自动化研究所_机器人学研究室
作者单位	1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China 2.University of Chinese Academy of Sciences, Beijing, 100049, China
推荐引用方式 GB/T 7714	Yang WG,Liu LQ,Wang YC,et al. Patterned microwell arrays for single-cell analysis & drug screening[C]. 见:1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017. Montreal, Canada. July 17-21, 2017.