In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae

doi:10.1007/s13238-016-0253-x

CORC > 地质与地球物理研究所 > 中国科学院地质与地球物理研究所 > 中国科学院地球与行星物理重点实验室

	In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae
	Deng, Aihua 1; Lin, Wei 2; Shi, Nana 1; Wu, Jie 1,3; Sun, Zhaopeng 1,3; Sun, Qinyun 1,3; Bai, Hua 1,3; Pan, Yongxin 2; Wen, Tingyi 1
刊名	PROTEIN & CELL
	2016-04-01
卷号	7 期号:4 页码:267-280
关键词	magnetotactic bacteria Nitrospirae bacterial actin MamK assembly mechanism
DOI	10.1007/s13238-016-0253-x
文献子类	Article
英文摘要	Magnetotactic bacteria (MTB), a group of phylogenetically diverse organisms that use their unique intracellular magnetosome organelles to swim along the Earth's magnetic field, play important roles in the biogeochemical cycles of iron and sulfur. Previous studies have revealed that the bacterial actin protein MamK plays essential roles in the linear arrangement of magnetosomes in MTB cells belonging to the Proteobacteria phylum. However, the molecular mechanisms of multiple-magnetosome-chain arrangements in MTB remain largely unknown. Here, we report that the MamK filaments from the uncultivated 'Candidatus Magnetobacterium casensis' (Mcas) within the phylum Nitrospirae polymerized in the presence of ATP alone and were stable without obvious ATP hydrolysis-mediated disassembly. MamK in Mcas can convert NTP to NDP and NDP to NMP, showing the highest preference to ATP. Unlike its Magnetospirillum counterparts, which form a single magnetosome chain, or other bacterial actins such as MreB and ParM, the polymerized MamK from Mcas is independent of metal ions and nucleotides except for ATP, and is assembled into well-ordered filamentous bundles consisted of multiple filaments. Our results suggest a dynamically stable assembly of MamK from the uncultivated Nitrospirae MTB that synthesizes multiple magnetosome chains per cell. These findings further improve the current knowledge of biomineralization and organelle biogenesis in prokaryotic systems.
WOS关键词	MAGNETOSPIRILLUM-MAGNETICUM AMB-1 ; MAGNETOTACTIC BACTERIA ; MAGNETOSOME FORMATION ; DYNAMIC INSTABILITY ; CELL-SHAPE ; MREB ; POLYMERIZATION ; DIVERSITY ; ORGANELLE ; CYTOSKELETON
WOS研究方向	Cell Biology
语种	英语
WOS记录号	WOS:000373638700004
资助机构	National Natural Science Foundation of China(31300065 ; National Natural Science Foundation of China(31300065 ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; 41330104) ; 41330104) ; National Natural Science Foundation of China(31300065 ; National Natural Science Foundation of China(31300065 ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; 41330104) ; 41330104)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/53457]
专题	地质与地球物理研究所_中国科学院地球与行星物理重点实验室
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Biogeomagnetism Grp, Paleomagnetism & Geochronol Lab, Key Lab Earth & Planetary Phys,Inst Geol & Geophy, Beijing 100029, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Deng, Aihua,Lin, Wei,Shi, Nana,et al. In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae[J]. PROTEIN & CELL,2016,7(4):267-280.
APA	Deng, Aihua.,Lin, Wei.,Shi, Nana.,Wu, Jie.,Sun, Zhaopeng.,...&Wen, Tingyi.(2016).In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae.PROTEIN & CELL,7(4),267-280.
MLA	Deng, Aihua,et al."In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae".PROTEIN & CELL 7.4(2016):267-280.