Magnetoferritin nanoparticles for targeting and visualizing tumour tissues

doi:10.1038/NNANO.2012.90

	Magnetoferritin nanoparticles for targeting and visualizing tumour tissues
	Fan, Kelong 1; Cao, Changqian 2; Pan, Yongxin 2; Lu, Di 1; Yang, Dongling 1; Feng, Jing 1; Song, Lina 1; Liang, Minmin 1; Yan, Xiyun 1
刊名	NATURE NANOTECHNOLOGY
	2012-07-01
卷号	7 期号:7 页码:459-464
ISSN号	1748-3387
DOI	10.1038/NNANO.2012.90
文献子类	Article
英文摘要	Engineered nanoparticles have been used to provide diagnostic(1-3), therapeutic(4,5) and prognostic information(6,7) about the status of disease. Nanoparticles developed for these purposes are typically modified with targeting ligands (such as antibodies(8-10), peptides(11,12) or small molecules(13)) or contrast agents(14-16) using complicated processes and expensive reagents. Moreover, this approach can lead to an excess of ligands on the nanoparticle surface, and this causes nonspecific binding(17-20) and aggregation of nanoparticles(18-20), which decreases detection sensitivity(17-20). Here, we show that magnetoferritin nanoparticles (M-HFn) can be used to target and visualize tumour tissues without the use of any targeting ligands or contrast agents. Iron oxide nanoparticles are encapsulated inside a recombinant human heavy-chain ferritin (HFn) protein shell, which binds to tumour cells that overexpress transferrin receptor 1 (TfR1). The iron oxide core catalyses the oxidation of peroxidase substrates in the presence of hydrogen peroxide to produce a colour reaction that is used to visualize tumour tissues. We examined 474 clinical specimens from patients with nine types of cancer and verified that these nanoparticles can distinguish cancerous cells from normal cells with a sensitivity of 98% and specificity of 95%.
WOS关键词	IRON-OXIDE NANOPARTICLES ; TRANSFERRIN RECEPTOR ; MULTIFUNCTIONAL NANOPARTICLES ; QUANTUM DOTS ; CANCER-CELLS ; FERRITIN ; PROTEIN ; EXPRESSION ; PEPTIDES ; BINDING
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000306112000014
资助机构	National Science and Technology Major Project(2012ZX10002009-016) ; National Science and Technology Major Project(2012ZX10002009-016) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; 973 Program(2011CB933500 ; 973 Program(2011CB933500 ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; 2012CB934003) ; 2012CB934003) ; National Science and Technology Major Project(2012ZX10002009-016) ; National Science and Technology Major Project(2012ZX10002009-016) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; 973 Program(2011CB933500 ; 973 Program(2011CB933500 ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; 2012CB934003) ; 2012CB934003) ; National Science and Technology Major Project(2012ZX10002009-016) ; National Science and Technology Major Project(2012ZX10002009-016) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; 973 Program(2011CB933500 ; 973 Program(2011CB933500 ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; 2012CB934003) ; 2012CB934003) ; National Science and Technology Major Project(2012ZX10002009-016) ; National Science and Technology Major Project(2012ZX10002009-016) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KJCX2-YW-M15) ; 973 Program(2011CB933500 ; 973 Program(2011CB933500 ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; National Defense Science and Technology Innovation Fund of Chinese Academy of Sciences(CXJJ-11-M61) ; 2012CB934003) ; 2012CB934003)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/84210]
专题	中国科学院地质与地球物理研究所
通讯作者	Fan, Kelong
作者单位	1.Chinese Acad Sci, Key Lab Prot & Peptide Pharmaceut, CAS Univ Tokyo Joint Lab Struct Virol & Immunol, Natl Lab Biomacromol,Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earths Deep Interior, Paleomagnetism & Geochronol Lab, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Fan, Kelong,Cao, Changqian,Pan, Yongxin,et al. Magnetoferritin nanoparticles for targeting and visualizing tumour tissues[J]. NATURE NANOTECHNOLOGY,2012,7(7):459-464.
APA	Fan, Kelong.,Cao, Changqian.,Pan, Yongxin.,Lu, Di.,Yang, Dongling.,...&Yan, Xiyun.(2012).Magnetoferritin nanoparticles for targeting and visualizing tumour tissues.NATURE NANOTECHNOLOGY,7(7),459-464.
MLA	Fan, Kelong,et al."Magnetoferritin nanoparticles for targeting and visualizing tumour tissues".NATURE NANOTECHNOLOGY 7.7(2012):459-464.