FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1

doi:10.1128/JB.00382-12

	FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1
	Rong, Chengbo 1,2,3; Zhang, Chan 1,2,4; Zhang, Yiting 1,2,4; Qi, Lei 1,2,4; Yang, Jing 1,2,4; Guan, Guohua 1,2,4; Li, Ying 1,2,4; Li, Jilun 1,2,4
刊名	JOURNAL OF BACTERIOLOGY
	2012-08-01
卷号	194 期号:15 页码:3972-3976
ISSN号	0021-9193
DOI	10.1128/JB.00382-12
文献子类	Article
英文摘要	Magnetotactic bacteria (MTB) synthesize unique organelles, the magnetosomes, which are intracellular nanometer-sized, membrane-enveloped magnetite. The biomineralization of magnetosomes involves the uptake of large amounts of iron. However, the iron metabolism of MTB is not well understood. The genome of the magnetotactic bacterium Magnetospirillum gryphiswaldense strain MSR-1 contains two ferrous iron transport genes, feoB1 and feoB2. The FeoB1 protein was reported to be responsible mainly for the transport of ferrous iron and to play an accessory role in magnetosome formation. To determine the role of feoB2, we constructed an feoB2 deletion mutant (MSR-1 Delta feoB2) and an feoB1 feoB2 double deletion mutant (MSR-1 NfeoB). The single feoB2 mutation did not affect magnetite crystal biomineralization. MSR-1 NfeoB had a significantly lower average magnetosome number per cell (similar to 65%) than MSR-1 Delta feoB1, indicating that FeoB2 plays a role in magnetosome formation when the feoB1 gene is deleted. Our findings showed that FeoB1 has a greater ferrous iron transport ability than FeoB2 and revealed the differential roles of FeoB1 and FeoB2 in MSR-1 iron metabolism. Interestingly, compared to the wild type, the feoB mutants showed increased sensitivity to oxidative stress and lower activities of the enzymes superoxide dismutase and catalase, indicating that the FeoB proteins help protect bacterial cells from oxidative stress.
WOS关键词	MAGNETICUM AMB-1 ; FERROUS IRON ; MAGNETOTACTIC BACTERIA ; TRANSPORT ; IDENTIFICATION ; MEMBRANE ; COMPLEX ; GROWTH
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000306634300021
资助机构	Chinese National Natural Science Foundation(30970041) ; Chinese National Natural Science Foundation(30970041) ; Innovation Program for Undergraduates from China Agricultural University ; Innovation Program for Undergraduates from China Agricultural University ; Chinese National Natural Science Foundation(30970041) ; Chinese National Natural Science Foundation(30970041) ; Innovation Program for Undergraduates from China Agricultural University ; Innovation Program for Undergraduates from China Agricultural University ; Chinese National Natural Science Foundation(30970041) ; Chinese National Natural Science Foundation(30970041) ; Innovation Program for Undergraduates from China Agricultural University ; Innovation Program for Undergraduates from China Agricultural University ; Chinese National Natural Science Foundation(30970041) ; Chinese National Natural Science Foundation(30970041) ; Innovation Program for Undergraduates from China Agricultural University ; Innovation Program for Undergraduates from China Agricultural University
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/84092]
专题	中国科学院地质与地球物理研究所
通讯作者	Li, Ying
作者单位	1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China 2.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing, Peoples R China 4.France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Rong, Chengbo,Zhang, Chan,Zhang, Yiting,et al. FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1[J]. JOURNAL OF BACTERIOLOGY,2012,194(15):3972-3976.
APA	Rong, Chengbo.,Zhang, Chan.,Zhang, Yiting.,Qi, Lei.,Yang, Jing.,...&Li, Jilun.(2012).FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1.JOURNAL OF BACTERIOLOGY,194(15),3972-3976.
MLA	Rong, Chengbo,et al."FeoB2 Functions in Magnetosome Formation and Oxidative Stress Protection in Magnetospirillum gryphiswaldense Strain MSR-1".JOURNAL OF BACTERIOLOGY 194.15(2012):3972-3976.