Fast on-Site Visual Detection of Active Ricin Using a Combination of Highly Efficient Dual-Recognition Affinity Magnetic Enrichment and a Specific Gold Nanoparticle Probe

doi:10.1021/acs.analchem.7b02944

	Fast on-Site Visual Detection of Active Ricin Using a Combination of Highly Efficient Dual-Recognition Affinity Magnetic Enrichment and a Specific Gold Nanoparticle Probe
	Sun, Jiefang 1,2; Wang, Cheng 1; Shao, Bing 1,2; Wang, Zhanhui 1; Xue, Dingshuai 3; Liu, Yanhong 3; Qi, Kailun 4; Yang, Yi 2; Niu, Yumin 2
刊名	ANALYTICAL CHEMISTRY
	2017-11-21
卷号	89 期号:22 页码:12209-12216
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b02944
文献子类	Article
英文摘要	Ricin, a highly toxic protein, is a controlled substance by both the Chemical Weapons Convention (CWC) and the Biological Weapons Convention (BWC). Therefore, fast precaution of potential ricin toxin plays an important role in national security and public safety. Herein, a simple, sensitive, and accurate visual detection of active ricin in complex samples is presented by combining magnetic affinity enrichment with a specific gold nanoparticle (AuNP) probe. In the first step, a dual-recognition magnetic absorbent was fabricated by simultaneously incorporating two different affinity ligands (concanavalin A and galactosamine) on low-foul polymer brushes grafted magnetic beads, which showed remarkable multivalent synergy binding capacity for ricin even under complex interfering environments. Subsequently, a homoadenine-constituted oligodeoxynucleotide named poly(21dA) was conjugated to AuNPs (the poly(21dA)-AuNPs), which served as a specific depurination substrate of active ricin. Coralyne can trigger the intact poly(21dA)-AuNPs aggregate by forming a non-Watson-Crick homoadenine/coralyne complex, but the poly(21dA)-AuNPs after reacting with active ricin failed to form this complex due to the loss of adenines. Based on these facts, active ricin can be detected as low as 12.5 ng mL(-1) with the naked eyes. This detection strategy could be well-applied in various ricin-spiked complex matrices. The features such as ready operation, facile readout, and easy accessibility make the assay a better choice for fast on-site active ricin detection.
WOS关键词	MASS-SPECTROMETRY ; FORENSIC IDENTIFICATION ; RAMAN-SCATTERING ; A-CHAIN ; PROTEIN ; DIGESTION ; TRYPSIN ; ASSAY ; QUANTIFICATION ; DEPURINATION
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000416498100045
资助机构	National Natural Science Foundation of China(21677019 ; National Natural Science Foundation of China(21677019 ; capital health and development of special(2014-1-3014) ; capital health and development of special(2014-1-3014) ; 21207142 ; 21207142 ; 41403021) ; 41403021) ; National Natural Science Foundation of China(21677019 ; National Natural Science Foundation of China(21677019 ; capital health and development of special(2014-1-3014) ; capital health and development of special(2014-1-3014) ; 21207142 ; 21207142 ; 41403021) ; 41403021) ; National Natural Science Foundation of China(21677019 ; National Natural Science Foundation of China(21677019 ; capital health and development of special(2014-1-3014) ; capital health and development of special(2014-1-3014) ; 21207142 ; 21207142 ; 41403021) ; 41403021) ; National Natural Science Foundation of China(21677019 ; National Natural Science Foundation of China(21677019 ; capital health and development of special(2014-1-3014) ; capital health and development of special(2014-1-3014) ; 21207142 ; 21207142 ; 41403021) ; 41403021)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/82040]
专题	中国科学院地质与地球物理研究所
通讯作者	Shao, Bing
作者单位	1.China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100193, Peoples R China 2.Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China 3.Chinese Acad Sci, Inst Geol & Geophys, State Key Lab Lithospher Evolut, Beijing 100029, Peoples R China 4.Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jiefang,Wang, Cheng,Shao, Bing,et al. Fast on-Site Visual Detection of Active Ricin Using a Combination of Highly Efficient Dual-Recognition Affinity Magnetic Enrichment and a Specific Gold Nanoparticle Probe[J]. ANALYTICAL CHEMISTRY,2017,89(22):12209-12216.
APA	Sun, Jiefang.,Wang, Cheng.,Shao, Bing.,Wang, Zhanhui.,Xue, Dingshuai.,...&Niu, Yumin.(2017).Fast on-Site Visual Detection of Active Ricin Using a Combination of Highly Efficient Dual-Recognition Affinity Magnetic Enrichment and a Specific Gold Nanoparticle Probe.ANALYTICAL CHEMISTRY,89(22),12209-12216.
MLA	Sun, Jiefang,et al."Fast on-Site Visual Detection of Active Ricin Using a Combination of Highly Efficient Dual-Recognition Affinity Magnetic Enrichment and a Specific Gold Nanoparticle Probe".ANALYTICAL CHEMISTRY 89.22(2017):12209-12216.