MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1

doi:10.1186/1471-2180-13-203

	MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1
	Yang,Jing 1,3; Li,Shuqi 1,3; Huang,Xiuliang 1,3; Li,Jinhua 2,3; Li,Li 1,3; Pan,Yongxin 2,3; Li,Ying 1,3
刊名	BMC Microbiology
	2013-09-11
卷号	13 期号:1
关键词	Magnetospirillum gryphiswaldense mamXY operon mamX Magnetosome Crystal maturation
ISSN号	1471-2180
DOI	10.1186/1471-2180-13-203
英文摘要	AbstractBackgroundMagnetotactic bacteria produce membrane-enveloped magnetite crystals (magnetosomes) whose formation is controlled primarily by a gene island termed the magnetosome island (MAI). Characterization of single gene and operon function in MAI has elucidated in part the genetic basis of magnetosome formation. The mamX gene, located in the mamXY operon, is highly conserved in the MAI of all Magnetospirillum strains studied to date. Little is known regarding the function of mamX in the process of biomineralization.ResultsA mamX deletion mutant (?mamX) and its complemented strain (CmamX) by conjugation in M. gryphiswaldense strain MSR-1 were constructed. There were no striking differences in cell growth among ?mamX, CmamX, and wild-type strain (WT). ?mamX displayed a much weaker magnetic response than WT. Transmission electron microscopy revealed the presence of irregular, superparamagnetic magnetite particles in ?mamX, in contrast to regular, single-domain particles in WT and CmamX. The phenotype of ?mamX was similar to that of an ftsZ-like deleted mutant and mamXY operon deleted mutant reported previously. Quantitative real-time RT-PCR (qPCR) results indicated that the deletion of mamX had differential effects on the transcription levels of the other three genes in the operon.ConclusionsThe MamX protein plays an important role in controlling magnetosome size, maturation, and crystal form. The four MamXY proteins appear to have redundant functions involved in magnetosome formation. Our findings provide new insights into the coordinated function of MAI genes and operons in magnetosome formation.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/1471-2180-13-203
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/64150]
专题	中国科学院地质与地球物理研究所
通讯作者	Li,Ying
作者单位	1.China Agricultural University; State Key Laboratories for Agro-biotechnology and College of Biological Sciences 2.Chinese Academy of Sciences; Institute of Geology and Geophysics 3.France-China Biomineralization and Nano-structure Laboratory
推荐引用方式 GB/T 7714	Yang,Jing,Li,Shuqi,Huang,Xiuliang,et al. MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1[J]. BMC Microbiology,2013,13(1).
APA	Yang,Jing.,Li,Shuqi.,Huang,Xiuliang.,Li,Jinhua.,Li,Li.,...&Li,Ying.(2013).MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1.BMC Microbiology,13(1).
MLA	Yang,Jing,et al."MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1".BMC Microbiology 13.1(2013).