The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments

doi:10.1007/s10811-016-0962-9

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 水质生物转化研究中心

	The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments
	Liu, Jing 1; Li, Zhe2 ; Guo, Jing-song1,2 ; Xiao, Yan2 ; Fang, Fang 1; Qin, Rong-cong 2; Zhang, Ling-ling 3
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2017-02-01
卷号	29 期号:1 页码:123-131
关键词	Chlorella sp. Critical nutrient supply ratio Critical cellular elemental ratio Light intensity Growth phase
ISSN号	0921-8971
DOI	10.1007/s10811-016-0962-9
通讯作者	Li, Z (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China.
英文摘要	Identification of nutrient status is essential to Chlorella sp. cultivation for biodiesel production. Critical cellular elemental ratio is a commonly used parameter to assess nutrient status, but the definition of critical cellular elemental ratio is inconsistent, and the influence of light and growth phase on this ratio is still unclear. In this study, Chlorella sp. was batch cultured under different conditions of light and nutrient supply ratio. Nitrate and phosphate consumption in the medium and particulate cellular carbon (C), nitrogen (N), and phosphorus (P) contents were measured. We first obtained the critical nutrient supply ratio to assess nutrient limitation and then utilized this ratio to deduce the critical cellular elemental ratio. The critical nutrient supply ratio was significantly affected by light intensity, and was approximately 45, 15, and between 30 to 45 under light intensities of 50, 100, and 200 mu mol photons m(-2) s(-1), respectively. Critical cellular C/N/P ratios of Chlorella sp. at three light intensities of 50, 100, and 200 mu mol photons m(-2) s(-1) in exponential growth phase were C159.91-237.30N15.90-22.52P, C104.02-167.39N11.71-17.35P, and C144.30-243.66N12.84-19.84P, and in stationary growth phase the ratios were C201.47-406.14N16.55-32.67P, C125.05-298.44N10.12-24.24P, and C168.65-342.42N14.47-29.18P, respectively. These results supported the hypothesis that the critical cellular elemental ratio, which is used to predict nutrient status, varies and depends on light intensity and growth phase.
资助项目	Chongqing Research Program of Basic Research and Frontier Technology[cstc2015jcyjBX0006] ; National Science Foundation of China[41430750] ; National Science Foundation of China[51309220] ; National Key S&T Project on Water Pollution Control and Treatment[2014ZX07104-006]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000396344800012
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/251]
专题	水质生物转化研究中心
通讯作者	Li, Zhe
作者单位	1.Chongqing Univ, Key Lab Gorges Reservoir Reg Ecoenvironm MOE, Chongqing 400045, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China 3.Xiamen Urban Planning & Design Inst, Municipal Dept, Xiamen 361000, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Li, Zhe,Guo, Jing-song,et al. The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments[J]. JOURNAL OF APPLIED PHYCOLOGY,2017,29(1):123-131.
APA	Liu, Jing.,Li, Zhe.,Guo, Jing-song.,Xiao, Yan.,Fang, Fang.,...&Zhang, Ling-ling.(2017).The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments.JOURNAL OF APPLIED PHYCOLOGY,29(1),123-131.
MLA	Liu, Jing,et al."The effect of light on the cellular stoichiometry of Chlorella sp. in different growth phases: implications of nutrient drawdown in batch experiments".JOURNAL OF APPLIED PHYCOLOGY 29.1(2017):123-131.