Metagenomic analysis of antibiotic resistance genes (ARGs) during refuse decomposition

doi:10.1016/j.scitotenv.2018.04.048

	Metagenomic analysis of antibiotic resistance genes (ARGs) during refuse decomposition
	Liu, Xi 1,2; Yang, Shu 3,4; Wang, Yangqing1 ; Zhao, He-Ping 5; Song, Liyan1
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2018-09-01
卷号	634 页码:1231-1237
关键词	Antibiotic resistance genes Metagenomics Refuse decomposition
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2018.04.048
英文摘要	Landfill is important reservoirs of residual antibiotics and antibiotic resistance genes (ARGs), but themechanism of landfill application influence on antibiotic resistance remains unclear. Although refuse decomposition plays a crucial role in landfill stabilization, its impact on the antibiotic resistance has not been well characterized. To better understand the impact, we studied the dynamics of ARGs and the bacterial community composition during refuse decomposition in a bench-scale bioreactor after long term operation (265 d) based onmetagenomics analysis. The total abundances of ARGs increased from431.0 ppmin the initial aerobic phase (AP) to 643.9 ppmin the latermethanogenic phase (MP) during refuse decomposition, suggesting that application of landfill formunicipal solid waste (MSW) treatment may elevate the level of ARGs. A shift from drug-specific (bacitracin, tetracycline and sulfonamide) resistance to multidrug resistance was observed during the refuse decomposition and was driven by a shift of potential bacteria hosts. The elevated abundance of Pseudomonas mainly contributed to the increasing abundance of multidrug ARGs (mexF and mexW). Accordingly, the percentage of ARGs encoding an efflux pump increased during refuse decomposition, suggesting that potential bacteria hosts developed this mechanism to adapt to the carbon and energy shortage when biodegradable substances were depleted. Overall, our findings indicate that the use of landfill forMSW treatment increased antibiotic resistance, and demonstrate the need for a comprehensive investigation of antibiotic resistance in landfill. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	Natural Science Foundation of China[51578528] ; Natural Science Foundation of China[51608506]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000433153600126
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/6479]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Song, Liyan
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Environm Microbiol & Ecol Res Ctr, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101407, Peoples R China 3.Univ Utah, Dept Geol, Salt Lake City, UT 84112 USA 4.Univ Utah, Dept Geophys, Salt Lake City, UT 84112 USA 5.Zhejiang Univ, Environm & Resource Sci, Hangzhou 310058, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xi,Yang, Shu,Wang, Yangqing,et al. Metagenomic analysis of antibiotic resistance genes (ARGs) during refuse decomposition[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2018,634:1231-1237.
APA	Liu, Xi,Yang, Shu,Wang, Yangqing,Zhao, He-Ping,&Song, Liyan.(2018).Metagenomic analysis of antibiotic resistance genes (ARGs) during refuse decomposition.SCIENCE OF THE TOTAL ENVIRONMENT,634,1231-1237.
MLA	Liu, Xi,et al."Metagenomic analysis of antibiotic resistance genes (ARGs) during refuse decomposition".SCIENCE OF THE TOTAL ENVIRONMENT 634(2018):1231-1237.