Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs) | |
Deng, Shun1,2; Jia, Pan-Pan2,3; Zhang, Jing-Hui2; Junaid, Muhammad2,3; Niu, Aping2; Ma, Yan-Bo2; Fu, Ailing1; Pei, De-Sheng1,2 | |
刊名 | JOURNAL OF HAZARDOUS MATERIALS |
2018-09-05 | |
卷号 | 357页码:146-158 |
关键词 | Gqds Signaling Pathways Transcriptome Analysis Zebrafish |
ISSN号 | 0304-3894 |
DOI | 10.1016/j.jhazmat.2018.05.063 |
英文摘要 | Graphene quantum dots (GQDs) are widely used for biomedical applications. Previously, the low-level toxicity of GQDs in vivo and in vitro has been elucidated, but the underlying molecular mechanisms remained largely unknown. Here, we employed the Illumina high-throughput RNA-sequencing to explore the whole-transcriptome profiling of zebrafish larvae after exposure to GQDs. Comparative transcriptome analysis identified 2116 differentially expressed genes between GQDs exposed groups and control. Functional classification demonstrated that a large proportion of genes involved in acute inflammatory responses and detoxifying process were significantly up-regulated by GQDs. The inferred gene regulatory network suggested that activator protein 1 (AP-1) was the early-response transcription factor in the linkage of a cascade of downstream (pro-) inflammatory signals with the apoptosis signals. Moreover, hierarchical signaling threshold determined the high sensitivity of complement system in zebrafish when exposed to the sublethal dose of GQDs. Further, 35 candidate genes from various signaling pathways were further validated by qPCR after exposure to 25, 50, and 100 mu g/mL of GQDs. Taken together, our study provided a valuable insight into the molecular mechanisms of potential bleeding risks and detoxifying processes in response to GQDs exposure, thereby establishing a mechanistic basis for the biosafety evaluation of GQDs. |
资助项目 | Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; Hundred Talents Program of Chinese Academy of Sciences ; National Natural Science Foundation of China[21407146] ; National Natural Science Foundation of China[51641907] |
WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000440958900016 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.138/handle/2HOD01W0/6553] |
专题 | 环境与健康研究中心 |
作者单位 | 1.Southwest Univ, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Deng, Shun,Jia, Pan-Pan,Zhang, Jing-Hui,et al. Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs)[J]. JOURNAL OF HAZARDOUS MATERIALS,2018,357:146-158. |
APA | Deng, Shun.,Jia, Pan-Pan.,Zhang, Jing-Hui.,Junaid, Muhammad.,Niu, Aping.,...&Pei, De-Sheng.(2018).Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs).JOURNAL OF HAZARDOUS MATERIALS,357,146-158. |
MLA | Deng, Shun,et al."Transcriptomic response and perturbation of toxicity pathways in zebrafish larvae after exposure to graphene quantum dots (GQDs)".JOURNAL OF HAZARDOUS MATERIALS 357(2018):146-158. |
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