Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy

doi:10.1039/c7ra09609g

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 太赫兹技术研究中心

	Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy
	Liu, Yu 1; Tang, Mingjie2 ; Xia, Liangping 2; Yu, Wenjing 1; Peng, Jia 1; Zhang, Yang 1; de la Chapelle, Marc Lamy 3; Yang, Ke 1; Cui, Hong-Liang2,4 ; Fu, Weiling 1
刊名	RSC ADVANCES
	2017
卷号	7 期号:85 页码:53963-53969
ISSN号	2046-2069
DOI	10.1039/c7ra09609g
英文摘要	As a fast-growing technology, terahertz time-domain spectroscopy (THz-TDS) is becoming increasingly pervasive in biological applications, targeting a range of biomaterials from biomolecules to tissues. However, THz-TDS studies at the cellular level are quite limited. Thus, a study to analyze the living state and hydration state of a tumor cell in a label-free manner is carried out and reported here. Combined with a specially designed THz metamaterial, a tumor cell monolayer was detected continuously over a period of time. In addition, in order to explore the possible impact of the metamaterial on tumor cells, the secretion of IL-6, IL-8, GM-CSF and GRO alpha of cell supernatants was detected. The results demonstrated that the technology could characterize the living state by monitoring the extracellular water and investigate the hydration state inside a tumor cell in real time, showing great application potential for the label-free detection of normal cells and tumor cells of diverse malignant degree.
资助项目	National Basic Research Program of China (Program 973) of China[2015CB755400] ; National Natural Science Foundation of China[81430054] ; Military Medical Pre-research Funds of The Third Military Medical University[SWH2013JS05] ; Physics and Biomedical Cross Laboratory Incubator Funds[WSS-2014-08]
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000416831000036
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/281]
专题	太赫兹技术研究中心
通讯作者	Cui, Hong-Liang; Fu, Weiling
作者单位	1.Third Mil Med Univ, Southwest Hosp, Dept Lab Med, Chongqing 400038, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 3.Univ Paris 13, CNRS, Lab CSPBAT, UMR 7244,Sorbonne Paris Cite, 74 Rue Marcel Cachin, F-93017 Bobigny, France 4.Jilin Univ, Coll Instrumentat Sci & Elect Engn, Changchun 130061, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yu,Tang, Mingjie,Xia, Liangping,et al. Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy[J]. RSC ADVANCES,2017,7(85):53963-53969.
APA	Liu, Yu.,Tang, Mingjie.,Xia, Liangping.,Yu, Wenjing.,Peng, Jia.,...&Fu, Weiling.(2017).Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy.RSC ADVANCES,7(85),53963-53969.
MLA	Liu, Yu,et al."Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy".RSC ADVANCES 7.85(2017):53963-53969.