Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages | |
Uddin, Md. Hemayet1; Wang, Huabin2; Rogerson, Fraser M.3,4; Lee, Peter Vee-Sin5; Zhang, Xuehua6 | |
刊名 | EUROPEAN PHYSICAL JOURNAL E |
2017-08-16 | |
卷号 | 40期号:8页码:1-7 |
ISSN号 | 1292-8941 |
DOI | 10.1140/epje/i2017-11561-1 |
英文摘要 | A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named "Jaffa". The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1 (IL-1) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1 treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques. |
资助项目 | National Natural Science Foundation of China[21407145] ; Chongqing Science and Technology Commission[cstc2015jcyjiA10057] ; Chongqing Science and Technology Commission[YJ500061LH1] ; Chinese Academy of Sciences[R52A500Z10] ; National Health and Medical Research Council of Australia |
WOS研究方向 | Chemistry ; Materials Science ; Physics ; Polymer Science |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000407635600001 |
内容类型 | 期刊论文 |
源URL | [http://172.16.51.4:88/handle/2HOD01W0/171] |
专题 | 太赫兹技术研究中心 |
作者单位 | 1.Victorian Node Australian Natl Fabricat Facil, Melbourne Cent Nanofabricat, Clayton, Vic 3149, Australia 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 3.Univ Melbourne, Dept Paediat, Parkville, Vic 3010, Australia 4.Royal Childrens Hosp, Murdoch Childrens Res Inst, Parkville, Vic 3010, Australia 5.Univ Melbourne, Dept Mech Engn, Parkville, Vic 3010, Australia 6.RMIT Univ, Sch Engn, Soft Matter & Interfaces Grp, Melbourne, Vic 3001, Australia |
推荐引用方式 GB/T 7714 | Uddin, Md. Hemayet,Wang, Huabin,Rogerson, Fraser M.,et al. Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages[J]. EUROPEAN PHYSICAL JOURNAL E,2017,40(8):1-7. |
APA | Uddin, Md. Hemayet,Wang, Huabin,Rogerson, Fraser M.,Lee, Peter Vee-Sin,&Zhang, Xuehua.(2017).Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages.EUROPEAN PHYSICAL JOURNAL E,40(8),1-7. |
MLA | Uddin, Md. Hemayet,et al."Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages".EUROPEAN PHYSICAL JOURNAL E 40.8(2017):1-7. |
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