Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish

doi:10.1016/j.biomaterials.2017.04.026

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 微纳制造与系统集成研究中心

	Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish
	Zhang, Jing-Hui 1,2; Sun, Tai1 ; Niu, Aping1 ; Tang, Yu-Mei 1; Deng, Shun 1; Luo, Wei 1; Xu, Qun 2; Wei, Dapeng1 ; Pei, De-Sheng1
刊名	BIOMATERIALS
	2017-07-01
卷号	133 页码:49-59
关键词	AhR pathway Bio-imaging Reduced graphene oxide quantum dots Real-time PCR Zebrafish
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2017.04.026
通讯作者	Wei, DP ; Pei, DS (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China. ; Xu, Q (reprint author), Zhengzhou Univ, Coll Mat Sci & Engn, Zhengzhou 450052, Peoples R China.
英文摘要	Graphene quantum dots (GQDs) has been widely used in enormous fields, however, the inherent molecular mechanism of GQDs for potential risks in biological system is still elusive to date. In this study, the outstanding reduced graphene quantum dots (rGOQDs) with the QY as high as 24.62% were successfully synthesized by the improved Hummers method and DMF hydrothermal treatment approach. The rGOQDs were N-doped photoluminescent nanomaterials with functional groups on the surface. The fluorescent bio-imaging was performed by exposing zebrafish in different concentrations of the as-prepared rGOQDs, and the distribution of rGOQDs was successfully observed. Moreover, the developmental toxicity and genotoxicity were evaluated to further investigate the potential hazard of rGOQDs. The result indicated that rGOQDs were responsible for the dose-dependent abnormalities on the development of zebrafish. Since the real-time polymerase chain reaction (RT-PCR) results showed that the expression of cyp1a was the highest expression in the selected genes and significantly up-regulated 8.49 fold in zebrafish, the perturbation of rGOQDs on aryl hydrocarbon receptor (AhR) pathway was investigated by using the Tg(cyp1a:gfp) zebrafish for the first time. The results demonstrated that rGOQDs significantly increased the green fluorescent protein (GFP) expression promoted by cyp1a in a dose-dependent manner, which was also further confirmed by the western blotting. This study offered an opportunity to reveal the potential hazards of in vivo bio-probes, which provided a valuable reference for investigating the graphene-based materials on the disturbance of AhR pathway in biological organisms. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Chinese Academy of Sciences ; Application and Development Program of Science and Technology Commission of Beibei District, Chongqing[2015-07] ; CAS ; Basic Scientific Research Program of Chongqing Science and Technology Commission[Cstc2016jcyjA0314]
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000401716700006
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/202]
专题	微纳制造与系统集成研究中心环境与健康研究中心
通讯作者	Xu, Qun; Wei, Dapeng; Pei, De-Sheng
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Zhengzhou Univ, Coll Mat Sci & Engn, Zhengzhou 450052, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jing-Hui,Sun, Tai,Niu, Aping,et al. Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish[J]. BIOMATERIALS,2017,133:49-59.
APA	Zhang, Jing-Hui.,Sun, Tai.,Niu, Aping.,Tang, Yu-Mei.,Deng, Shun.,...&Pei, De-Sheng.(2017).Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish.BIOMATERIALS,133,49-59.
MLA	Zhang, Jing-Hui,et al."Perturbation effect of reduced graphene oxide quantum dots (rGOQDs) on aryl hydrocarbon receptor (AhR) pathway in zebrafish".BIOMATERIALS 133(2017):49-59.