The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP

doi:10.1371/journal.pone.0155762

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 环境与健康研究中心

	The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP
	Jia, Pan-Pan; Ma, Yan-Bo; Lu, Chun-Jiao; Mirza, Zakaria; Zhang, Wei; Jia, Yong-Fang; Li, Wei-Guo; Pei, De-Sheng
刊名	PLOS ONE
	2016
卷号	11 期号:5
ISSN号	1932-6203
DOI	10.1371/journal.pone.0155762
通讯作者	Li, WG (reprint author), Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China. ; Pei, DS (reprint author), Chinese Acad Sci, Res Ctr Environm & Hlth, Chongqing Inst Green & Intelligent Technol, Ecoenvironm Inst Three Gorges, Chongqing 401122, Peoples R China.
英文摘要	Di-(2-ethylhexyl) phthalate (DEHP) has the potential to disrupt the thyroid endocrine system, but the underlying mechanism is unknown. In this study, zebrafish (Danio rerio) embryos were exposed to different concentrations of DEHP (0, 40, 100, 200, 400 mu g/L) from 2 to 168 hours post fertilization (hpf). Thyroid hormones (THs) levels and transcriptional profiling of key genes related to hypothalamus-pituitary-thyroid (HPT) axis were examined. The result of whole-body thyroxine (T4) and triiodothyronine (T3) indicated that the thyroid hormone homeostasis was disrupted by DEHP in the zebrafish larvae. After exposure to DEHP, the mRNA expressions of thyroid stimulating hormone (tsh beta) and corticotrophin releasing hormone (crh) genes were increased in a concentration dependent manner, respectively. The expression level of genes involved in thyroid development (nkx2.1 and pax8) and thyroid synthesis (sodium/iodide symporter, nis, thyroglobulin, tg) were also measured. The transcripts of nkx2.1 and tg were significantly increased after DEHP exposure, while those of nis and pax8 had no significant change. Down-regulation of uridinediphosphate-glucuronosyl-transferase (ugt1ab) and up-regulation of thyronine deiodinase (dio2) might change the THs levels. In addition, the transcript of transthyretin (ttr) was up-regulated, while the mRNA levels of thyroid hormone receptors (tr alpha and tr beta) remained unchanged. All the results demonstrated that exposure to DEHP altered the whole-body thyroid hormones in the zebrafish larvae and changed the expression profiling of key genes related to HPT axis, proving that DEHP induced the thyroid endocrine toxicity and potentially affected the synthesis, regulation and action of thyroid hormones.
语种	英语
WOS记录号	WOS:000376881700028
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/4331]
专题	环境与健康研究中心科研公共服务平台
作者单位	(1) Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China; (2) Chinese Acad Sci, Res Ctr Environm & Hlth, Chongqing Inst Green & Intelligent Technol, Ecoenvironm Inst Three Gorges, Chongqing 401122, Peoples R China
推荐引用方式 GB/T 7714	Jia, Pan-Pan,Ma, Yan-Bo,Lu, Chun-Jiao,et al. The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP[J]. PLOS ONE,2016,11(5).
APA	Jia, Pan-Pan.,Ma, Yan-Bo.,Lu, Chun-Jiao.,Mirza, Zakaria.,Zhang, Wei.,...&Pei, De-Sheng.(2016).The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP.PLOS ONE,11(5).
MLA	Jia, Pan-Pan,et al."The Effects of Disturbance on Hypothalamus-Pituitary-Thyroid (HPT) Axis in Zebrafish Larvae after Exposure to DEHP".PLOS ONE 11.5(2016).