Low-Concentration Serum Promotes the Adhesion and Spreading of Mouse Fibroblast L929 Cell on Hydrophobic Glass Surface

doi:10.1166/jbt.2017.1572

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	Low-Concentration Serum Promotes the Adhesion and Spreading of Mouse Fibroblast L929 Cell on Hydrophobic Glass Surface
	Wang, Weiping 1; Wang, Rong 2; Qin, Peng 3; Yang, Huan 2; Ma, Changguo 2; Zhang, Fan 2; Zhang, Qiao 1; Cui, Yuehua3 ; Zhang, Huidong 2
刊名	JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
	2017-04-01
卷号	7 期号:4 页码:333-342
关键词	Hydrophobic Surface Surface Energy Mouse L929 Cell Adhesion Spreading Serum Concentration
ISSN号	2157-9083
DOI	10.1166/jbt.2017.1572
通讯作者	Zhang, Q (reprint author), Zhengzhou Univ, Dept Toxicol, Coll Publ Hlth, Zhengzhou 450001, Henan, Peoples R China. ; Zhang, HD (reprint author), Third Mil Med Univ, Inst Toxicol, Coll Prevent Med, Chongqing 400030, Peoples R China. ; Cui, YH (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China.
英文摘要	The effective utilization of biomaterials in regenerative medicine and tissue engineering critically relies on the understanding of the intricate interactions between cells and biomaterials. The surface energy of biomaterials affects various cellular functions, but this mechanism is unclear. How to improve cell functions on the hydrophobic surface is a promising approach for practical application. In this study, we modified a glass slide surface with inert alkyl groups having different chain lengths. The surfaces had only polar and nonpolar surface energies but no charged or reactive groups. The adhesion, spreading, and proliferation of mouse L929 cell and the absorption of serum protein were studied on these surfaces. The reduction in polar surface energy inhibited these cell behaviors. The nonpolar surface energy appeared to determine protein adsorption and affinity on the surface, and the polar surface energy may stabilize protein conformation. Cell adhesion and spreading on the hydrophobic surface can be significantly improved by decreasing serum concentrations. This improvement can be effectively applied in biomedicine and tissue engineering.
资助项目	Natural Science Foundation of China (NSFC)[31370793] ; Natural Science Foundation of China (NSFC)[814220410] ; Natural Science Foundation of China (NSFC)[21473180] ; Youth 1000 Talent Plan
WOS研究方向	Cell Biology
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000398928800010
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/3589]
专题	科研公共服务平台
通讯作者	Zhang, Qiao; Cui, Yuehua; Zhang, Huidong
作者单位	1.Zhengzhou Univ, Dept Toxicol, Coll Publ Hlth, Zhengzhou 450001, Henan, Peoples R China 2.Third Mil Med Univ, Inst Toxicol, Coll Prevent Med, Chongqing 400030, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weiping,Wang, Rong,Qin, Peng,et al. Low-Concentration Serum Promotes the Adhesion and Spreading of Mouse Fibroblast L929 Cell on Hydrophobic Glass Surface[J]. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,2017,7(4):333-342.
APA	Wang, Weiping.,Wang, Rong.,Qin, Peng.,Yang, Huan.,Ma, Changguo.,...&Zhang, Huidong.(2017).Low-Concentration Serum Promotes the Adhesion and Spreading of Mouse Fibroblast L929 Cell on Hydrophobic Glass Surface.JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,7(4),333-342.
MLA	Wang, Weiping,et al."Low-Concentration Serum Promotes the Adhesion and Spreading of Mouse Fibroblast L929 Cell on Hydrophobic Glass Surface".JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 7.4(2017):333-342.