Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions
	Wang, Xuewei 1,2; Ding, Jiawang1 ; Song, Wenjing 1,2; Xie, Kang 1,3; Qin, Wei1
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2012-01-03
卷号	161 期号:1 页码:1119-1123
关键词	Potentiometry Ion-selective Electrode Polyion Protamine Heparin Electrode Conditioning
ISSN号	0925-4005
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Chunhui Rd 17, Yantai 264003, Peoples R China. wqin@yic.ac.cn
产权排序	[Wang, Xuewei; Ding, Jiawang; Song, Wenjing; Xie, Kang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Xuewei; Song, Wenjing] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xie, Kang] Yantai Univ, Coll Chem & Biol, Yantai 264005, Peoples R China
文献子类	Article
英文摘要	A novel operation mode for development of sensitive and renewable polymeric membrane polyion-sensitive electrodes has been explored in this paper. In contrast to the traditional polyion sensors which are conditioned with highly discriminated ions, the proposed polycation-sensitive membrane electrode with dinonylnaphthalene sulfonate as the ion-exchanger is conditioned with the primary ion (protamine), and activated with the discriminated ion (Na+) before measurement. Using this protocol, the membrane with protamine in the membrane bulk but with discriminated ions rich at the membrane outer surface can be prepared. The protamine inside the membrane efficiently suppresses the fluxes of protamine from the sample-membrane interface into the membrane bulk, thus decreasing the detection limit toward protamine by one order of magnitude. With the reproducible protamine fluxes obtained by the conditioning and activation steps, the protamine-sensitive electrode could be reused without significant signal deterioration. Titrimetric analysis of heparin with this novel technique has been demonstrated. The proposed polyion sensing platform offers much promise in the detection of polyions as well as in other polyion-involved bioanalyses.; A novel operation mode for development of sensitive and renewable polymeric membrane polyion-sensitive electrodes has been explored in this paper. In contrast to the traditional polyion sensors which are conditioned with highly discriminated ions, the proposed polycation-sensitive membrane electrode with dinonylnaphthalene sulfonate as the ion-exchanger is conditioned with the primary ion (protamine), and activated with the discriminated ion (Na+) before measurement. Using this protocol, the membrane with protamine in the membrane bulk but with discriminated ions rich at the membrane outer surface can be prepared. The protamine inside the membrane efficiently suppresses the fluxes of protamine from the sample-membrane interface into the membrane bulk, thus decreasing the detection limit toward protamine by one order of magnitude. With the reproducible protamine fluxes obtained by the conditioning and activation steps, the protamine-sensitive electrode could be reused without significant signal deterioration. Titrimetric analysis of heparin with this novel technique has been demonstrated. The proposed polyion sensing platform offers much promise in the detection of polyions as well as in other polyion-involved bioanalyses. (c) 2011 Elsevier B.V. All rights reserved.
学科主题	Chemistry ; Electrochemistry ; Instruments & Instrumentation
URL标识	查看原文
WOS关键词	RESPONSE CHARACTERISTICS ; ELECTROCHEMICAL ASSAY ; REVERSIBLE DETECTION ; HEPARIN ; PROTAMINE ; SENSORS ; POTENTIOMETRY ; POLYANIONS ; BINDING
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000301549400153
资助机构	National Natural Science Foundation of China[41176081]; Outstanding Youth Natural Science Foundation of Shandong Province[JQ200814]; Taishan Scholar Program of Shandong Province[TS20081159]
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5532]
专题	烟台海岸带研究所_近岸生态与环境实验室
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Yantai Univ, Coll Chem & Biol, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuewei,Ding, Jiawang,Song, Wenjing,et al. Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions[J]. SENSORS AND ACTUATORS B-CHEMICAL,2012,161(1):1119-1123.
APA	Wang, Xuewei,Ding, Jiawang,Song, Wenjing,Xie, Kang,&Qin, Wei.(2012).Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions.SENSORS AND ACTUATORS B-CHEMICAL,161(1),1119-1123.
MLA	Wang, Xuewei,et al."Primary-ion-conditioned polymeric membrane electrodes for sensitive detection of polyions".SENSORS AND ACTUATORS B-CHEMICAL 161.1(2012):1119-1123.