Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics
	Liu, Xiaoli 1,2; Zhang, Linbao 1,2; You, Liping 1,2; Yu, Junbao1 ; Zhao, Jianmin1 ; Li, Lianzhen1 ; Wang, Qing1 ; Li, Fei1 ; Li, Chenghua 1; Liu, Dongyan1
刊名	ECOTOXICOLOGY
	2011
卷号	20 期号:1 页码:177-186
关键词	Manila Clam Bioindicator Mercury Nmr Metabolomics
通讯作者	Wu, HF, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China.hfwu@yic.ac.cn
产权排序	[Liu, Xiaoli; Zhang, Linbao; You, Liping; Yu, Junbao; Zhao, Jianmin; Li, Lianzhen; Wang, Qing; Li, Fei; Li, Chenghua; Liu, Dongyan; Wu, Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China; [Liu, Xiaoli; Zhang, Linbao; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Mercury is a hazardous pollutant in the Bohai marine environments due to its high toxicity to the marine organisms and subsequent ecological risk. Manila clam Ruditapes philippinarum is one of important sentinel organisms in 'Mussel Watch Program' launched in China and therefore used as a bioindicator in marine and coastal ecotoxicology. There are dominantly distributed three pedigrees of clam (White, Liangdao Red and Zebra) in Yantai population endowed with different tolerances to environmental stressors. In this study, gill tissues were collected from both untreated and mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the original metabolomes and the toxicological effects induced by mercury exposure in three pedigrees. The major abundant metabolites in White clam sample were branched-chain amino acids, lactate, alanine, arginine, acetoacetate, glutamate, succinate, citrate, malonate and taurine, while the metabolite profile of Liangdao Red clam sample comprises relative high levels of alanine, arginine, glutamate, succinate and glycogen. For Zebra clam sample, the metabolite profile exhibited relatively high amount of aspartate, acetylcholine and homarine. After 48 h exposure of 20 mu g l(-1) Hg2+, the metabolic profiles from all the three pedigrees of clams commonly showed significant increases in alanine, arginine, glutamate, aspartate, alpha-ketoglutarate, glycine and ATP/ADP, and decreases in citrate, taurine and homarine. The unique metabolic differences between the metabolomes of gill tissues from Hg2+-exposed White, Liangdao Red and Zebra clams were found, including elevated acetylcholine and branched-chain amino acids in White clams, and the declined succinate in both White and Liangdao Red samples as well as the declined betaine in Zebra and White clams. Overall, our findings showed the differential toxicological responses to mercury exposure and that White clams could be a preferable bioindicator for the metal pollution monitoring based on the metabolic changes from gill compared with other two (Liangdao Red and Zebra) pedigrees of clams.; Mercury is a hazardous pollutant in the Bohai marine environments due to its high toxicity to the marine organisms and subsequent ecological risk. Manila clam Ruditapes philippinarum is one of important sentinel organisms in 'Mussel Watch Program' launched in China and therefore used as a bioindicator in marine and coastal ecotoxicology. There are dominantly distributed three pedigrees of clam (White, Liangdao Red and Zebra) in Yantai population endowed with different tolerances to environmental stressors. In this study, gill tissues were collected from both untreated and mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the original metabolomes and the toxicological effects induced by mercury exposure in three pedigrees. The major abundant metabolites in White clam sample were branched-chain amino acids, lactate, alanine, arginine, acetoacetate, glutamate, succinate, citrate, malonate and taurine, while the metabolite profile of Liangdao Red clam sample comprises relative high levels of alanine, arginine, glutamate, succinate and glycogen. For Zebra clam sample, the metabolite profile exhibited relatively high amount of aspartate, acetylcholine and homarine. After 48 h exposure of 20 mu g l(-1) Hg2+, the metabolic profiles from all the three pedigrees of clams commonly showed significant increases in alanine, arginine, glutamate, aspartate, alpha-ketoglutarate, glycine and ATP/ADP, and decreases in citrate, taurine and homarine. The unique metabolic differences between the metabolomes of gill tissues from Hg2+-exposed White, Liangdao Red and Zebra clams were found, including elevated acetylcholine and branched-chain amino acids in White clams, and the declined succinate in both White and Liangdao Red samples as well as the declined betaine in Zebra and White clams. Overall, our findings showed the differential toxicological responses to mercury exposure and that White clams could be a preferable bioindicator for the metal pollution monitoring based on the metabolic changes from gill compared with other two (Liangdao Red and Zebra) pedigrees of clams.
学科主题	Ecology ; Environmental Sciences ; Toxicology
WOS关键词	H-1-NMR METABOLOMICS ; PATTERN-RECOGNITION ; OXIDATIVE STRESS ; TIDAL FLATS ; SPECTROSCOPY ; METABONOMICS ; EXTRACTION ; ALANINE ; TISSUE ; LA(NO3)(3)
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
WOS记录号	WOS:000286003700019
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/4890]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_滨海湿地实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics[J]. ECOTOXICOLOGY,2011,20(1):177-186.
APA	Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Zhao, Jianmin.,...&Wu, Huifeng.(2011).Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics.ECOTOXICOLOGY,20(1),177-186.
MLA	Liu, Xiaoli,et al."Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics".ECOTOXICOLOGY 20.1(2011):177-186.