Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure
	Li, Chenghua 1,2,3; Li, Lingyun 4; Liu, Feng 5; Ning, Xuanxuan 6; Chen, Aiqin 1,2; Zhang, Linbao 1,2; Wu, Huifeng1,2 ; Zhao, Jianmin1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2011
卷号	30 期号:1 页码:447-450
关键词	Venerupis Philippinarum Hsp40 Vibrio Anguillarum Cu(2+) Cd(2+)
ISSN号	1050-4648
通讯作者	Zhao, JM, CAS, Key Lab Coastal Zone Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.jmzhao@yic.ac.cn
产权排序	[Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Wu, Huifeng; Zhao, Jianmin] CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Li, Chenghua] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Li, Lingyun] NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Yantai 264670, Peoples R China; [Ning, Xuanxuan] Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai 264006, Peoples R China
文献子类	Article
英文摘要	HSP40 was an understudied protein family with co-chaperone activity. In the present study, a HSP40 homology was cloned from Venerupis philippinarum haemocytes (designated as VpHSP40) by EST analysis and RACE approaches. The expression profiles of VpHSP40 under Vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression, and the highest expression level was detected at 24 h post-infection with 6.0-fold increase compared with that in the control group. During heavy metal exposure experiment, the expression of VpHSP40 could also be induced by Cu2+ and Cd2+ at different concentration, where Cu2+ displayed more toxic effect on clam than that of Cd2+. Concerning the same heavy metal, varied effect on VpHSP40 expression was detected at different concentration of heavy metal. Taking together, these results suggested that VpHSP40 was perhaps involved in mediating the immune responses and environmental stresses in V. philippinarum. (C) 2010 Elsevier Ltd. All rights reserved.; HSP40 was an understudied protein family with co-chaperone activity. In the present study, a HSP40 homology was cloned from Venerupis philippinarum haemocytes (designated as VpHSP40) by EST analysis and RACE approaches. The expression profiles of VpHSP40 under Vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression, and the highest expression level was detected at 24 h post-infection with 6.0-fold increase compared with that in the control group. During heavy metal exposure experiment, the expression of VpHSP40 could also be induced by Cu(2+) and Cd(2+) at different concentration, where Cu(2+) displayed more toxic effect on clam than that of Cd(2+). Concerning the same heavy metal, varied effect on VpHSP40 expression was detected at different concentration of heavy metal. Taking together, these results suggested that VpHSP40 was perhaps involved in mediating the immune responses and environmental stresses in V. philippinarum. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS关键词	MESSENGER-RNA EXPRESSION ; SHOCK-PROTEIN 40 ; MOLECULAR CHAPERONES ; HSP70 GENE ; CELLS ; CLONING ; STRESS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000286905800058
公开日期	2011-07-22
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/4884]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.CAS, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 4.NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China 5.China Agr Univ Yantai, Yantai 264670, Peoples R China 6.Yantai Ocean Environm Monitoring Cent Stn SOA, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Li, Chenghua,Li, Lingyun,Liu, Feng,et al. Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):447-450.
APA	Li, Chenghua.,Li, Lingyun.,Liu, Feng.,Ning, Xuanxuan.,Chen, Aiqin.,...&Zhao, Jianmin.(2011).Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure.FISH & SHELLFISH IMMUNOLOGY,30(1),447-450.
MLA	Li, Chenghua,et al."Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):447-450.