Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii

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	Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii
	Zhang Liping 1,2; Shao Hongbo 1,2,3; Long Xiaohua 1; Liu Zhaopu 1
刊名	PLOS ONE
	2013-01-22
卷号	8 期号:1 页码:e53835
关键词	Phytochrome-chromophore Biosynthesis Nitric-oxide Metal Transporter Effector Molecules Chelate Reductase Arabidopsis Plants Accumulation Metabolism Expression
ISSN号	1932-6203
通讯作者	Shao, HB (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China. shaohongbochu@126.com ; longxiaohua@njau.edu.cn ; sea@njau.edu.cn
产权排序	[Zhang Liping; Shao Hongbo; Long Xiaohua; Liu Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China; [Zhang Liping; Shao Hongbo] Chinese Acad Sci, Yantai Inst Costal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China; [Shao Hongbo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao, Peoples R China
文献子类	Article
英文摘要	Carbon monoxide (CO) as an endogenous gaseous molecule regulates a variety of biological processes in animals. However, CO regulating nutrient stress responses in green alga is largely unknown. On the other hand, heme oxydase (HO1 as a rate-limiting enzyme of the first step for heme degration and to catalyze heme into biliverdin (BV), which is concomitant with releasing of CO and ferrous ions, probably participates in the process of CO-regulating response to nutrient stress in green alga. In this paper, we described an observation that CO could regulate iron-homeostasis in iron-starving Chlamydomonas reinhardtii. Exogenous CO at 8 mu M was able to prevent the iron deficient-inducing chlorosis and improve chlorophyll accumulation. Expression pattern of FOX1, FTR1 and ferredoxin was up-regulated by CO exposure in iron-deficient mediam. treatment with external CO increasing iron accumulation in iron-deficient C. reinhardtii. Moreover, to get insights into the regulatory role of HO1, we constructed a transgenic alga overexpressing HO1 and HO1 knock-out mutants. The results show that there was no significant influence on chlorosis with HO1 overexpression of C. reinhardtii under iron-deficiency and the chlorophyll accumulation, and gene expression associated with iron deficiency of mutant were greatly improved. Otherwise, those results from HO1 knock-out mutants were opposite to HO1 overexpression mutants. Finally, CO exposure induced NO accumulation in cells. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO/HO1 may play an important role in improving green algae adaptation to iron deficiency or cross-talking with NO under the iron deficiency.; Carbon monoxide (CO) as an endogenous gaseous molecule regulates a variety of biological processes in animals. However, CO regulating nutrient stress responses in green alga is largely unknown. On the other hand, heme oxydase (HO1 as a rate-limiting enzyme of the first step for heme degration and to catalyze heme into biliverdin (BV), which is concomitant with releasing of CO and ferrous ions, probably participates in the process of CO-regulating response to nutrient stress in green alga. In this paper, we described an observation that CO could regulate iron-homeostasis in iron-starving Chlamydomonas reinhardtii. Exogenous CO at 8 mu M was able to prevent the iron deficient-inducing chlorosis and improve chlorophyll accumulation. Expression pattern of FOX1, FTR1 and ferredoxin was up-regulated by CO exposure in iron-deficient mediam. treatment with external CO increasing iron accumulation in iron-deficient C. reinhardtii. Moreover, to get insights into the regulatory role of HO1, we constructed a transgenic alga overexpressing HO1 and HO1 knock-out mutants. The results show that there was no significant influence on chlorosis with HO1 overexpression of C. reinhardtii under iron-deficiency and the chlorophyll accumulation, and gene expression associated with iron deficiency of mutant were greatly improved. Otherwise, those results from HO1 knock-out mutants were opposite to HO1 overexpression mutants. Finally, CO exposure induced NO accumulation in cells. However, such an action could be blocked by NO scavenger cPTIO. These results indicate that CO/HO1 may play an important role in improving green algae adaptation to iron deficiency or cross-talking with NO under the iron deficiency.
学科主题	Multidisciplinary Sciences
URL标识	查看原文
WOS关键词	PHYTOCHROME-CHROMOPHORE BIOSYNTHESIS ; NITRIC-OXIDE ; METAL TRANSPORTER ; EFFECTOR MOLECULES ; CHELATE REDUCTASE ; ARABIDOPSIS ; PLANTS ; ACCUMULATION ; METABOLISM ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000314019100033
资助机构	National Key Project of Scientific and Technical Supporting Programs [2011BAD13B09]; Jiangsu Provincial Key Project of Scientific and Technical Supporting Programs [BE2010305, BE2011368]; Fundamental Research Funds for the Central Universities [Y0201100249]; New Teachers' Fund for Doctor Stations, Ministry of Education [20100097120016]; Project of Special Fund for Public Welfare Industrial (Agriculture) Research of China [200903001-5]; National Natural Science Foundation of China [31201692]; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS); CAS/SAFEA International Partnership Program for Creative Research Teams; Yantai Science & Technology Development Project [2011016, 2010245]; Yantai Double-hundred Talent Plan [XY-003-02]; 135 Plan Development Plan of YIC-CAS
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6439]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Key Lab Marine Biol, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Costal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China 3.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhang Liping,Shao Hongbo,Long Xiaohua,et al. Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii[J]. PLOS ONE,2013,8(1):e53835.
APA	Zhang Liping,Shao Hongbo,Long Xiaohua,&Liu Zhaopu.(2013).Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii.PLOS ONE,8(1),e53835.
MLA	Zhang Liping,et al."Gene Regulation of Iron-Deficiency Responses Is Associated with Carbon Monoxide and Heme Oxydase 1 in Chlamydomonas reinhardtii".PLOS ONE 8.1(2013):e53835.