Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803

doi:10.3390/en10122093

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	Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803
	Chen, Gao 1,2; Chen, Jun 3,4; He, Qingfang 5; Zhang, Yan 1,2; Peng, Zhenying 1,2; Fan, Zhongxue 1,2; Bian, Fei 1,2; Yu, Jinhui 1,2; Qin, Song3 ; Chen, Gao(Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China)
刊名	ENERGIES
	2017-12-01
卷号	10 期号:12
关键词	Fatty Acids Gene Expression Acyl-acyl Carrier Protein Thioesterases Synechocystis Sp Pcc6803
ISSN号	1996-1073
DOI	10.3390/en10122093
产权排序	第3完成单位
文献子类	Article
英文摘要	Palmitoleic acid (C16:1) and stearic acid (C18:0) are precursors of polyunsaturated fatty acids, which are the focus of intensive global research due to their nutritional value, medicinal applications, and potential use as biofuel. Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that determine the types and amounts of fatty acids produced in plants and release fatty acids into the cytosol to be incorporated into glycerolipids. Based on amino acid sequence identity and substrate specificity, these enzymes are classified into two families, FatA and FatB. In this study, we cloned FatA and FatB thioesterases from Arachis hypogaea L. seeds and functionally expressed these genes, both individually and in tandem, in a blue-green alga Synechocystis sp. PCC6803. The heterologous expression of these genes in Synechocystis altered the fatty acid composition of lipids, resulting in a 29.5-31.6% increase in palmitoleic acid production and a 22.5-35.5% increase in stearic acid production. Moreover, the transgenic Synechocystis cells also showed significant increases in levels of oleic acid (C18: 1, OA), linoleic acid (C18: 2, LA), and alpha-linolenic acid (C18: 3n3, ALA). These results suggest that the fatty acid profile of algae can be significantly improved by the heterologous expression of exogenous genes. This study not only provides insight into fatty acid biosynthesis, but also lays the foundation for manipulating the fatty acid content of cyanobacteria.
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WOS关键词	CARRIER PROTEIN THIOESTERASE ; BIODIESEL PRODUCTION ; TRANSGENIC PLANTS ; CYANOBACTERIA ; PURIFICATION ; MICROALGAE ; CLONING ; ALGAE ; SEEDS ; GENE
WOS研究方向	Energy & Fuels
语种	英语
WOS记录号	WOS:000423156900164
资助机构	Natural Science Foundation of Shandong Province, China(ZR2016CM48) ; Young Talents Training Program of Shandong Academy of Agricultural Sciences ; China Postdoctoral Science Foundation(2014M551942) ; National Key Research and Development Program-China(2016YFF0202304)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/23566]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Chen, Gao; Qin, Song; Chen, Gao(Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China); Qin, Song(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China)
作者单位	1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China 2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA
推荐引用方式 GB/T 7714	Chen, Gao,Chen, Jun,He, Qingfang,et al. Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803[J]. ENERGIES,2017,10(12).
APA	Chen, Gao.,Chen, Jun.,He, Qingfang.,Zhang, Yan.,Peng, Zhenying.,...&Qin, Song.(2017).Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803.ENERGIES,10(12).
MLA	Chen, Gao,et al."Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803".ENERGIES 10.12(2017).