Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara
	Liu, XF; Wu, YH; Wei, SN; Wang, N; Li, PF; Li, YZ; Zhang, NW; Qin, QW; Chen, SL
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2018
卷号	44 期号:1 页码:87-93
通讯作者	chensl@ysfri.ac.cn
英文摘要	A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 A degrees C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 A degrees C, with optimal growth at 24 A degrees C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42. Green fluorescent signals could be observed in EMK cells when the cells were transfected with pEGFP-N3 plasmid. Moreover, a significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or nervous necrosis virus (NNV), and viral replication was confirmed by quantitative real-time PCR (qPCR). These results suggested the potential of the EMK cell line for studies of transgene and pathogenesis of SGIV and NNV.
学科主题	Biochemistry & Molecular Biology; Fisheries; Physiology
关键词[WOS]	Kidney cell line ; Epinephelus moara ; Transfection ; SGIV ; NNV
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17012]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Liu, XF,Wu, YH,Wei, SN,et al. Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(1):87-93.
APA	Liu, XF.,Wu, YH.,Wei, SN.,Wang, N.,Li, PF.,...&Chen, SL.(2018).Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(1),87-93.
MLA	Liu, XF,et al."Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.1(2018):87-93.