Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara
	Liu, XF; Wu, YH; Wei, SN; Wang, N; Li, YZ; Zhang, NW; Li, PF; Qin, QW; Chen, SL
刊名	JOURNAL OF FISH BIOLOGY
	2018
卷号	92 期号:2 页码:298-307
通讯作者	chensl@ysfri.ac.cn
英文摘要	A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30 degrees C, with the maximum growth between 24 and 30 degrees C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45. After being transfected with pEGFP-N3 plasmid, the cells could successfully express green fluorescence protein (GFP), implying that this cell line can be used for transgenic studies. A significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV) and the viral replication was confirmed by quantitative real-time PCR (qrt-PCR) assay, which suggested EMB's application potential for studies of SGIV and RGNNV.
学科主题	Fisheries; Marine & Freshwater Biology
关键词[WOS]	brain-cell line ; GFP ; LCDV ; NNV ; primary culture ; SGIV
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17008]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Liu, XF,Wu, YH,Wei, SN,et al. Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara[J]. JOURNAL OF FISH BIOLOGY,2018,92(2):298-307.
APA	Liu, XF.,Wu, YH.,Wei, SN.,Wang, N.,Li, YZ.,...&Chen, SL.(2018).Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara.JOURNAL OF FISH BIOLOGY,92(2),298-307.
MLA	Liu, XF,et al."Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara".JOURNAL OF FISH BIOLOGY 92.2(2018):298-307.