A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi
	Cheng, CH; Tian, YS; Chen, T; Luo, P; Huang, W; Huo, D; Yun, L; Hu, CQ
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018
卷号	78 页码:364-371
通讯作者	luopeng@scsio.ac.cn
英文摘要	Fibrinogen-related proteins (FREPs) play a crucial role in invertebrate immune response. In this study, we acquired a novel fibrinogen-related protein gene in Litopenaeus vannamei coding for one kind of fibrinogen-related protein, designated as LvFREP2. The complete cDNA sequence of LvFREP2 was 1903 bp long, containing an open reading frame of 1479 bp coding for LvFREP2. The LvFREP2 protein contained a putative signal peptide and a fibrinogen-related protein domain. qRT-PCRs indicated that LvFREP2 mRNA ubiquitously distributed in all examined tissues, and it was up-regulated in gills after V. harveyi and LPS challenges. The recombinant LvFREP2 agglutinated Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Vibrio alginolyticus, V. cholerae, V. vulnificus, V. parahaemolyticus, V. harveyi, Pseudomonas aeruginosa, P. fluorescens) in a calcium-dependent manner. LvFREP2 also facilitated the clearance of Vibrio harveyi in vivo. Therefore, our results suggested that lvFREP2 may have important roles in the anti-bacterial immunity of L. vannamei.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
关键词[WOS]	Litopenaeus vannamei ; Fibrinogen-related proteins ; Vibrio harveyi ; Innate immunity
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/16924]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Cheng, CH,Tian, YS,Chen, T,et al. A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi[J]. FISH & SHELLFISH IMMUNOLOGY,2018,78:364-371.
APA	Cheng, CH.,Tian, YS.,Chen, T.,Luo, P.,Huang, W.,...&Hu, CQ.(2018).A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi.FISH & SHELLFISH IMMUNOLOGY,78,364-371.
MLA	Cheng, CH,et al."A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi".FISH & SHELLFISH IMMUNOLOGY 78(2018):364-371.