Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip | |
Zhai, Zhimin1,2; Zhang, Fengqiu1,2,3; Chen, Xiangyu2; Zhong, Jie1,2; Liu, Gang2; Tian, Yangchao2; Huang, Qing1,2 | |
刊名 | LAB ON A CHIP |
2017-04-07 | |
卷号 | 17期号:7页码:1306-1313 |
DOI | 10.1039/c7lc00053g |
文献子类 | Article |
英文摘要 | This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way. |
WOS关键词 | LABEL-FREE DETECTION ; FOLATE RECEPTOR EXPRESSION ; GOLD NANOPARTICLES ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; MOLECULAR RECOGNITION ; SERS NANOPARTICLES ; OVARIAN-CANCER ; SINGLE-CELL ; IN-VIVO |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
语种 | 英语 |
WOS记录号 | WOS:000398955800010 |
资助机构 | National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/33355] |
专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Zhai, Zhimin,Zhang, Fengqiu,Chen, Xiangyu,et al. Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip[J]. LAB ON A CHIP,2017,17(7):1306-1313. |
APA | Zhai, Zhimin.,Zhang, Fengqiu.,Chen, Xiangyu.,Zhong, Jie.,Liu, Gang.,...&Huang, Qing.(2017).Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.LAB ON A CHIP,17(7),1306-1313. |
MLA | Zhai, Zhimin,et al."Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip".LAB ON A CHIP 17.7(2017):1306-1313. |
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