Development of dual-fluorescence cell-based biosensors for detecting the influence of environmental factors on nanoparticle toxicity

doi:10.1016/j.chemosphere.2016.12.076

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Development of dual-fluorescence cell-based biosensors for detecting the influence of environmental factors on nanoparticle toxicity
	Liang, Junting 1,2,3; Wang, Xuanyu 1,3; Li, Luzhi 1,3; Xu, Shengmin 1,3; Jiang, Jiang 4,5; Wu, Lijun 1,3; Zhao, Guoping 1,3; Chen, Shaopeng 1,3
刊名	CHEMOSPHERE
	2017-03-01
卷号	171 期号:无页码:177-184
关键词	Biosensor Fluorescent Protein Nanoparticles Environmental Factor Flow Cytometry
DOI	10.1016/j.chemosphere.2016.12.076
文献子类	Article
英文摘要	With the expanding use of engineered nanoparticles (NPs), development of a high-throughput, sensitive method for evaluating NP safety is important. In this study, we developed cell-based biosensors to efficiently and conveniently monitor NP toxicity. The biosensor cells were obtained by transiently transfecting human cells with biosensor plasmids containing a mCherry gene regulated by an inducible promoter [an activator protein 1 (AP-1) promoter, an interleukin 8 (IL8) promoter, or a B cell translocation gene 2 (BTG2) promoter], with an enhanced green-fluorescent protein gene driven by the cytomegalovirus promoter as the internal control. After optimizing flow cytometric analysis, these dual-fluorescence cell-based biosensors were capable of accurately and rapidly detecting NP toxicity. We found that the responses of AP-1, BTG2, and IL8 biosensors in assessing the toxicity of silver nanoparticles (Ag NPs) showed good dose-related increases after exposure to Ag NPs and were consistent with data acquired by conventional assays, such as western blot, real-time polymerase chain reaction, and immunofluorescence. Further investigation of the effects of environmental factors on Ag NP toxicity revealed that aging in water, co-exposure with fulvic acid, and irradiation with ultraviolet A light could affect Ag NP-induced biosensor responses. These results indicated that these novel dual-fluorescence biosensors can be applied to accurately and sensitively monitor NP toxicity. (C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词	TITANIUM-DIOXIDE NANOPARTICLES ; DNA-DAMAGE ; OXIDATIVE STRESS ; CANCER-CELLS ; ASSAY ; GENOTOXICITY ; PROBE ; NANOTECHNOLOGY ; AGGREGATION ; CHALLENGES
WOS研究方向	Environmental Sciences & Ecology
语种	英语
WOS记录号	WOS:000393931100021
资助机构	Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; Strategic Leading Science & Technology Program(XDB14030502) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; CASHIPS director's fund(Y411201501) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044) ; China Postdoctorial Science Foundation(2016M602044)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/32835]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China 3.Key Lab Environm Toxicol & Pollut Control Technol, Hefei, Anhui, Peoples R China 4.Univ Nebraska Med Ctr, Dept Surg Transplant, Omaha, NE 68198 USA 5.Univ Nebraska Med Ctr, Mary & Dick Holland Regenerat Med Program, Omaha, NE 68198 USA
推荐引用方式 GB/T 7714	Liang, Junting,Wang, Xuanyu,Li, Luzhi,et al. Development of dual-fluorescence cell-based biosensors for detecting the influence of environmental factors on nanoparticle toxicity[J]. CHEMOSPHERE,2017,171(无):177-184.
APA	Liang, Junting.,Wang, Xuanyu.,Li, Luzhi.,Xu, Shengmin.,Jiang, Jiang.,...&Chen, Shaopeng.(2017).Development of dual-fluorescence cell-based biosensors for detecting the influence of environmental factors on nanoparticle toxicity.CHEMOSPHERE,171(无),177-184.
MLA	Liang, Junting,et al."Development of dual-fluorescence cell-based biosensors for detecting the influence of environmental factors on nanoparticle toxicity".CHEMOSPHERE 171.无(2017):177-184.