Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris

doi:10.1007/s10295-017-1931-2

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris
	Li, Zhemin 1; Zhao, Genhai 1; Liu, Hui 1; Guo, Yugang 2; Wu, Hefang 1; Sun, Xiaowen 1; Wu, Xihua 1; Zheng, Zhiming 1
刊名	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
	2017-07-01
卷号	44 期号:7 页码:973-985
关键词	Aromatic Prenyltransferase Pichia Pastoris Menadione Prenylation Chemoenzymatic Synthesis Whole Cell Catalysis
DOI	10.1007/s10295-017-1931-2
文献子类	Article
英文摘要	Prenylated quinones, especially menaquinones, have significant physiological activities, but are arduous to synthesize efficiently. Due to the relaxed aromatic substrate specificity and prenylation regiospecificity at the ortho- site of the phenolic hydroxyl group, the aromatic prenyltransferase NovQ from Streptomyces may be useful in menaquinone synthesis from menadione. In this study, NovQ was overexpressed in Pichia pastoris. After fermentation optimization, NovQ production increased by 1617%. Then the different effects of metal ions, detergents and pH on the activity of purified NovQ were investigated to optimize the prenylation reaction. Finally, purified NovQ and cells containing NovQ were used for menadione prenylation in vitro and in vivo, respectively. Menaquinone-1 (MK-1) was detected as the only product in vitro with gamma,gamma-dimethylallyl pyrophosphate and menadione hydroquinol substrates. MK-3 at a concentration of 90.53 mg/L was detected as the major product of whole cell catalysis with 3-methyl-2-buten-1-ol and menadione hydroquinol substrates. This study realized whole cell catalysis converting menadione to menaquinones.
WOS关键词	DETERGENT-SUPPLEMENTED CULTURE ; FLAVOBACTERIUM SP 238-7 ; VITAMIN-K ; ESCHERICHIA-COLI ; MENAQUINONE ; BIOSYNTHESIS ; ENZYMES ; PROTEIN ; PRENYLTRANSFERASES ; MENINGOSEPTICUM
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000403589300001
资助机构	Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07) ; Natural Science Foundation of Anhui Province(1308085MA07)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/32016]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci & Anhui Prov, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Innovat Ctr Cell Biol, CAS Key Lab Innate Immun & Chron Dis, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhemin,Zhao, Genhai,Liu, Hui,et al. Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2017,44(7):973-985.
APA	Li, Zhemin.,Zhao, Genhai.,Liu, Hui.,Guo, Yugang.,Wu, Hefang.,...&Zheng, Zhiming.(2017).Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,44(7),973-985.
MLA	Li, Zhemin,et al."Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 44.7(2017):973-985.