Optimization Medium Composition for Vitamin K-2 by Flavobacterium sp using Response Surface Methodology and Addition of Arachis hypogaea

doi:10.1590/1678-4324-2016150343

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Optimization Medium Composition for Vitamin K-2 by Flavobacterium sp using Response Surface Methodology and Addition of Arachis hypogaea
	Tan, Mu; Liu, Hui; Li, Zhemin; Sun, Xiaowen; Zheng, Zhiming; Zhao, Genhai
刊名	BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
	2016
卷号	59 期号:无页码:1-13
关键词	Arachis Hypogaea D-(+)-catechin Response Surface Methodology Vitamin K-2
DOI	10.1590/1678-4324-2016150343
文献子类	Article
英文摘要	The purpose of this research was to enhance the production of vitamin K-2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37 degrees C with initial pH value 7.0 +/- 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM), optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K(2)HPO(4)4.5 g/l, NaCl 3.0 g/l and MgSO4 center dot 7H(2)O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l). 3 mg/mL of arachis hypogaea was added into the medium at 72 h of shake-flake cultivation, which improved the production of menaquinone-4 (MK4) up to 371% and menaquinone-6 (MK6) up to 149% higher than those of the original medium. D-(+)-catechin, one of the components of arachis hypogaea, was added alone into the medium, which also improved the vitamin K-2 synthesis.
WOS关键词	PROCYANIDIN A1 ; MUTANT ; MENAQUINONE ; EXPRESSION
WOS研究方向	Life Sciences & Biomedicine - Other Topics
语种	英语
WOS记录号	WOS:000376478900035
资助机构	key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; key 863 Fund of China(2014AA021704) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22417]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Tan, Mu,Liu, Hui,Li, Zhemin,et al. Optimization Medium Composition for Vitamin K-2 by Flavobacterium sp using Response Surface Methodology and Addition of Arachis hypogaea[J]. BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,2016,59(无):1-13.
APA	Tan, Mu,Liu, Hui,Li, Zhemin,Sun, Xiaowen,Zheng, Zhiming,&Zhao, Genhai.(2016).Optimization Medium Composition for Vitamin K-2 by Flavobacterium sp using Response Surface Methodology and Addition of Arachis hypogaea.BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY,59(无),1-13.
MLA	Tan, Mu,et al."Optimization Medium Composition for Vitamin K-2 by Flavobacterium sp using Response Surface Methodology and Addition of Arachis hypogaea".BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY 59.无(2016):1-13.