The Nogo-B receptor promotes Ras plasma membrane localization and activation

doi:10.1038/onc.2016.484

	The Nogo-B receptor promotes Ras plasma membrane localization and activation
	Zhao, B.1,2,6; Hu, W.1,6; Kumar, S.1; Gonyo, P.4; Rana, U.1,6; Liu, Z.1,6; Wang, B.1,3,6; Duong, W. Q.1,6; Yang, Z.5; Williams, C. L.4
刊名	ONCOGENE
	2017-06-15
卷号	36 页码:3406-3416
DOI	10.1038/onc.2016.484
英文摘要	The localization of prenylated Ras at the plasma membrane promotes activation of Ras by receptor tyrosine kinases and stimulates oncogenic signaling by mutant Ras. The Nogo-B receptor (NgBR) is a transmembrane receptor that contains a conserved hydrophobic pocket. Here, we demonstrate that the NgBR promotes the membrane accumulation of Ras by directly binding prenylated Ras at the plasma membrane. We show that NgBR knockdown diminishes the membrane localization of Ras in multiple cell types. NgBR overexpression in NIH-3T3 fibroblasts increases membrane-associated Ras, induces the transformed phenotype in vitro, and promotes the formation of fibrosarcoma in nude mice. NgBR knockdown in human breast cancer cells reduces Ras membrane localization, inhibits epidermal growth factor (EGF)-stimulated Ras signaling and diminishes tumorigenesis of xenografts in nude mice. Our data demonstrate that NgBR is a unique receptor that promotes accumulation of prenylated Ras at the plasma membrane and promotes EGF pathways.
语种	英语
WOS记录号	WOS:000403340700005
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152096]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Med Coll Wisconsin, Dept Pathol, Childrens Res Inst, Div Pediat Pathol, Milwaukee, WI 53226 USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci, Dalian, Liaoning, Peoples R China 3.China Japan Friendship Hosp, Dept Pathol, Beijing, Peoples R China 4.Med Coll Wisconsin, Dept Pharmacol & Toxicol, Milwaukee, WI 53226 USA 5.Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI USA 6.Med Coll Wisconsin, Dept Surg, Childrens Res Inst, Div Pediat Surg, 8700 W Wisconsin Ave, Milwaukee, WI 53226 USA
推荐引用方式 GB/T 7714	Zhao, B.,Hu, W.,Kumar, S.,et al. The Nogo-B receptor promotes Ras plasma membrane localization and activation[J]. ONCOGENE,2017,36:3406-3416.
APA	Zhao, B..,Hu, W..,Kumar, S..,Gonyo, P..,Rana, U..,...&Miao, Q. R..(2017).The Nogo-B receptor promotes Ras plasma membrane localization and activation.ONCOGENE,36,3406-3416.
MLA	Zhao, B.,et al."The Nogo-B receptor promotes Ras plasma membrane localization and activation".ONCOGENE 36(2017):3406-3416.