A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging

doi:10.1039/c7nj02391j

	A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging
	Guo, Lan 1; Yang, Daoshan 1; Xia, Lian 1; Qu, Fei 1; Dou, Yanan 1; Qu, Fengli 1; Kong, Rongmei 1; You, Jinmao1,2
刊名	NEW JOURNAL OF CHEMISTRY
	2017-12-21
卷号	41 期号:24 页码:15277-15282
DOI	10.1039/c7nj02391j
英文摘要	In this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells.
语种	英语
WOS记录号	WOS:000417058800063
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13284]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Guo, Lan,Yang, Daoshan,Xia, Lian,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017,41(24):15277-15282.
APA	Guo, Lan.,Yang, Daoshan.,Xia, Lian.,Qu, Fei.,Dou, Yanan.,...&You, Jinmao.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY,41(24),15277-15282.
MLA	Guo, Lan,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY 41.24(2017):15277-15282.