Selective and sensitive detection of Zn(II) ion using a simple peptide-based sensor

doi:10.1016/j.snb.2017.08.038

	Selective and sensitive detection of Zn(II) ion using a simple peptide-based sensor
	Wan, Jiajia 1; Duan, Wenxiu 1; Chen, Kai 1; Tao, Yanduo 2; Dang, Jun 2; Zeng, Kehao 3; Ge, Yushu 1; Wu, Jiang 3; Liu, Dan 1
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-02-01
卷号	255 页码:49-56
关键词	Peptide-based Sensor Zinc Ion detectIon Fluorescence Emission Bio-imaging
DOI	10.1016/j.snb.2017.08.038
英文摘要	The development of biocompatible chemosensors is becoming increasingly important for chemical analyses in environmental sampling, the food industry, and clinical medicine. Many current chemosensors have limited utility as they represent potential secondary pollutants. To circumvent this problem, researchers are evaluating peptide-based fluorescent chemosensors because of their well-established synthesis protocols, good biological compatibility, and generally low cytotoxicity, which makes them suitable for bioimaging. In this study, we report the development of a peptide-based fluorescent chemosensor HL (fluorophore-Cys-Pro-Gly-His) for divalent zinc ions (Zn2+). The objective of this study was to select a suitable peptide sequence and associated fluorophore exhibiting high selectivity and sensitivity to Zn2+-binding, and enhanced fluorescent response. Results of this study, both in vitro and in living cells, indicated that HL exhibited high selectivity and sensitivity for Zn2+, with a limit of detection (LOD) of about 82 nM. The sensor also exhibited very low cell cytotoxicity and strong fluorescence emission in living cells, which indicates that it may find application as a novel chemosensor for selective Zn2+ detection in biological environments. (C) 2017 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000414151800007
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/10364]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Collaborat Innovat Ctr Chem Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 3.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wan, Jiajia,Duan, Wenxiu,Chen, Kai,et al. Selective and sensitive detection of Zn(II) ion using a simple peptide-based sensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:49-56.
APA	Wan, Jiajia.,Duan, Wenxiu.,Chen, Kai.,Tao, Yanduo.,Dang, Jun.,...&Liu, Dan.(2018).Selective and sensitive detection of Zn(II) ion using a simple peptide-based sensor.SENSORS AND ACTUATORS B-CHEMICAL,255,49-56.
MLA	Wan, Jiajia,et al."Selective and sensitive detection of Zn(II) ion using a simple peptide-based sensor".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):49-56.