| A universal strategy for aptamer-based nanopore sensing through host-guest interactions inside -hemolysin | |
| Li, Ting; Liu, Lei; Li, Yuru; Xie, Jiani; Wu, Hai-Chen | |
| 刊名 | Angewandte chemie-international edition
 |
| 2015-06-22 | |
| 卷号 | 54期号:26页码:7568-7571 |
| 关键词 | Aptamers Host-guest systems Magnetic beads Nanopore sensors Quantitative determination |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201502047 |
| 英文摘要 | Nanopore emerged as a powerful single-molecule technique over the past two decades, and has shown applications in the stochastic sensing and biophysical studies of individual molecules. here, we report a versatile strategy for nanopore sensing by employing the combination of aptamers and host-guest interactions. an aptamer is first hybridized with a dna probe which is modified with a ferrocene< subset of>cucurbit[7]uril complex. the presence of analytes causes the aptamer-probe duplex to unwind and release the dna probe which can quantitatively produce signature current events when translocated through an -hemolysin nanopore. the integrated use of magnetic beads can further lower the detection limit by approximately two to three orders of magnitude. because aptamers have shown robust binding affinities with a wide variety of target molecules, our proposed strategy should be universally applicable for sensing different types of analytes with nanopore sensors. |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000356390900018 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/1639378 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Wu, Hai-Chen |
| 作者单位 | Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ting,Liu, Lei,Li, Yuru,et al. A universal strategy for aptamer-based nanopore sensing through host-guest interactions inside -hemolysin[J]. Angewandte chemie-international edition,2015,54(26):7568-7571. |
| APA | Li, Ting,Liu, Lei,Li, Yuru,Xie, Jiani,&Wu, Hai-Chen.(2015).A universal strategy for aptamer-based nanopore sensing through host-guest interactions inside -hemolysin.Angewandte chemie-international edition,54(26),7568-7571. |
| MLA | Li, Ting,et al."A universal strategy for aptamer-based nanopore sensing through host-guest interactions inside -hemolysin".Angewandte chemie-international edition 54.26(2015):7568-7571. |
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